Lysate (1.5% SDS / 100 mm Tris-Cl) was added to samples on ice, mixed well and centrifuged to obtain supernatant. The protein concentration was determined by Bradford assay, and the protein bands were visualized by SDS-PAGE. Enzymatic digestion with trypsin was followed by Sep-Pak C18 desalting. Equ...
ORGANISM(S): Homo sapiens (Human)
