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GenomicsHigh-Resolution Nucleosome Mapping Reveals Transcription-Dependent Promoter Packaging
Genome-wide mapping of nucleosomes has revealed a great deal about the relationships between chromatin structure and control of gene expression, and has led to mechanistic hypotheses regarding the rules by which chromatin structure is established. High-throughput sequencing has recently become the t...
ORGANISM(S): Saccharomyces cerevisiae 
2009-10-15 | E-GEOD-18530 | biostudies-arrayexpress
TranscriptomicsHigh-resolution view of the yeast meiotic program revealed by ribosome profiling
Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured mRNA abundance and protein production through yeast sporulation and found strong temporal control for most genes, achieved through both mRNA levels and translational regulation. Monitoring t...
ORGANISM(S): Saccharomyces cerevisiae 
2012-01-06 | E-GEOD-34082 | biostudies-arrayexpress
TranscriptomicsComprehensive comparative analysis of strand-specific RNA sequencing methods
Strand-specific massively-parallel cDNA sequencing (RNA-Seq) is a powerful tool for novel transcript discovery, genome annotation, and expression profiling. Despite multiple published methods for strand-specific RNA-Seq, no consensus exists as to how to choose between them. Here, we developed a co...
ORGANISM(S): Saccharomyces cerevisiae 
2010-08-15 | E-GEOD-21739 | biostudies-arrayexpress
TranscriptomicsRNA Polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast
The use of genome-wide RNA abundance profiling by microarrays and deep sequencing has spurred a revolution in our understanding of transcriptional control. However, changes in mRNA abundance reflect the combined effect of changes in RNA production, processing, and degradation, and thus, mRNA levels ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-07-16 | E-GEOD-22675 | biostudies-arrayexpress
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