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TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in matrigel or a non-adhesive alginate hydrogel after 28 days of in vitro growth. Additionally, we used scRNA-seq to profile HIOs derived in the presence of Neureguli...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10268 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of pluripotent stem cell derived human intestinal organoids, adult intestine, and human fetal endoderm derived organs
Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human definitive endoderm and intestinal organoids (HIOs) at several timepoints of in vitro growth (7, 14, and 28 days) and after in vivo growth beneath the kidney capsule of a murine host (4 and 8 wks post...
ORGANISM(S): Homo sapiens 
2021-05-21 | E-MTAB-10187 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestinal ileum tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal ileum tissue samples from 3 individual biological specimens ages 11.4, 14.4, and 18.9 weeks post conception. The data set is composed of over 16,000 cells from diverse intestinal lineages.
ORGANISM(S): Homo sapiens 
2021-02-22 | E-MTAB-9906 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal duodenal enteroids grown in EGF and/or NRG1
The purpose of this study was to interrogate the role of EGF-family ligands, in particular Epidermal Growth Factor (EGF) and Neuregulin 1 (NRG1), as human intestinal stem cell niche factors. An established duodenal enteroid line derived from a 20.2 week post conception biological specimen was expose...
ORGANISM(S): Homo sapiens 
2020-12-10 | E-MTAB-9720 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal intestine tissue
Here, we performed single cell RNA sequencing (scRNA-seq) of human fetal duodenal tissue samples from 8 individual biological specimens across 7-21 weeks of gestation. The data set is composed of over 37,000 cells (between 3,000-9,000 cells per time point) from diverse intestinal lineages. Lineages ...
ORGANISM(S): Homo sapiens 
2020-12-12 | E-MTAB-9489 | biostudies-arrayexpress
TranscriptomicsEpithelium-only cultured stem cells isolated from human pluripotent stem cell derived intestinal organoids grown in matrigel and alginate
Human intestinal organoids were grown in a typical 3D matrigel culture environment, or in an alginate gel, then a subset from each condition were xenotransplanted to vascularized and mature in vivo. Epithelium was isolated and epithelial only organoids (enteroids) were then grown from each condition...
ORGANISM(S): Homo sapiens 
2019-06-27 | E-MTAB-7000 | biostudies-arrayexpress
TranscriptomicsMultiomic (Joint snRNA/snATAC) sequencing data of intestinal crypts and epithelial only organoids derived from human fetal duodenum
Here, we used joint single-nuclei RNA-sequencing (snRNA-seq) and single-nuclei ATAC sequencing (scATAC) to profile freshly isolated crypts from the human fetal intestine and matched intestinal epithelial only organoids (also known as enteroids) derived from these crypts after one passage of in vitro...
ORGANISM(S): Homo sapiens 
2022-07-16 | E-MTAB-11908 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of intestinal epithelial organoids derived from human fetal duodenum
Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of EGF or 1 ng/ml of EREG added.
ORGANISM(S): Homo sapiens 
2022-07-17 | E-MTAB-11912 | biostudies-arrayexpress
TranscriptomicsSingle cell RNA sequencing of iPSC-derived lung organoid models
Here, we used single cell RNA sequencing of iPSC17 WT 7B2-derived day 10 lung spheroids, unsorted 3-, 6-, and 10-week lung progenitor organoids (LPOs) and induced bud tip progenitor organoids (iBTOs) sorted from 4- and 10-week LPOs, sequenced 4 weeks post-sort. All cultures were grown in serum-free ...
ORGANISM(S): Homo sapiens 
2022-07-24 | E-MTAB-11953 | biostudies-arrayexpress
TranscriptomicsscRNA-seq of human fetal lung explants with R-SPONDIN Inhibition and human fetal lung primary tissue
Here, we used single cell RNA-sequencing (scRNA-seq) to profile 13-week post-conception distal human fetal lung explants cultured in an air liquid interface system and treated with an LGR5 ectodomain adenovirus that inhibits R-Spondin function or control adenovirus. A third, non-infected, condition ...
ORGANISM(S): Homo sapiens 
2022-01-01 | E-MTAB-10662 | biostudies-arrayexpress
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