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Diadenosine polyphosphates (ApnAs) are noncanonical nucleotides that are ubiquitous across all forms of life. Due to their strong accumulation upon cellular stress, they are considered alarmones to signal homeostatic imbalance. Nonetheless, their cellular role is poorly understood. In this work, we ...
ORGANISM(S): Homo sapiens (Human) 
2023-01-27 | PXD036450 | Pride
Rhizobium leguminosarum biovar viciae strain 3841 was grown on glucose ammonia AMS versus acetate ammonia AMS and gene expression compared
ORGANISM(S): Rhizobium leguminosarum 
Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.
ORGANISM(S): Rhizobium leguminosarum 
Time course of bacteroid formation. Bacteroids were harvested from pea nodules at 7, 15 and 21 d after inoculation onto 7 d old pea plants. The transcription profile of each bacteroid sample at the 3 time points was compared to cells grown in free-living culture on succinate ammonia AMS.
ORGANISM(S): Rhizobium leguminosarum 
Free-living cultures of Rhizobium leguminosarum strain 3841 grown on pyruvate ammonia AMS vesus the same strain grown on glucose ammonia AMS.
ORGANISM(S): Rhizobium leguminosarum 
Rhizobium leguminosarum biovar viciae strain 3841 was grown in AMS minimal salts on either glucose ammonia or myo-inositol ammonia and gene expression compared.
ORGANISM(S): Rhizobium leguminosarum 
RNA ligases are present across all forms of life. While enzymatic RNA ligation between 5'-PO4 and 3'-OH termini is prevalent in viruses, fungi, and plants, such RNA ligases are yet to be identified in vertebrates. Here, using a nucleotide-based chemical probe targeting human AMPylated proteome, we h...
ORGANISM(S): Homo sapiens (Human) 
2023-01-27 | PXD038132 | Pride
Free-living bacteria were grown on succinae ammonia AMS and gene expression was compared to free-living bacteria grown on glucose ammonia AMS.
ORGANISM(S): Rhizobium leguminosarum 
Rhizobium leguminosarum bivar viciae strain 3841 was grown on AMS minimal medium with either succinate ammonia or glucose ammonia as carbon and nitrogen sources. Gene expression was compared between the two crabon sources. Total RNA was amplified using Genispheres SenseAmp kit.
ORGANISM(S): Rhizobium leguminosarum 
Rhizobium leguminosarum biovar viciae strain 3841 was inoculated onto Viccia cracca seeds and bacteroids were harvested at 28d. Gene expression was then compared to free-living Rhizobium leguminosarum biovar viciae strain 3841 grown on succinate ammonia AMS.
ORGANISM(S): Rhizobium leguminosarum 
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