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TranscriptomicsThe transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program
Mature lymphoid cells express the transcriptional repressor Bach2, which imposes regulation on humoral and cellular immunity. Here we found critical roles for Bach2 in the development of cells of the B lineage, commencing from the common lymphoid progenitor (CLP) stage, with Bach1 as an auxiliary. O...
ORGANISM(S): Mus musculus 
2014-10-26 | E-GEOD-61409 | biostudies-arrayexpress
ProteomicsLFQ of GFP-Trap IP-MS from BDKO cells expressing Tmem63b-GFP
Cell lysates were prepared from Tmem63b-GFP-expressing BDKO cells and incubated with the GFP-Trap magnetic agarose beads (Proteintech). After rotation at 4˚C for 3 hr, the beads were precipitated with the magnet, followed by washing several times using wash buffer containing 25 mM Tris-HCl (pH 8.0),...
ORGANISM(S): Mus Musculus (mouse) 
2024-08-21 | PXD054885 | JPOST Repository
ProteomicsHuman serum proteome of low-dose radiation area
Two sampling areas were focused in this study; Tande tande hamlet as high-level natural background radiation area (HBRA) and Topoyo sub-district as normal level background radiation area (NBRA), which was done under coordination with Local Health Office. The former located in 2° 44’ 38.3’’ south lat...
ORGANISM(S): Homo Sapiens (human) 
2022-05-12 | PXD025946 | JPOST Repository
ProteomicsLFQ of Spot-Trap IP-MS from Y-79 cells expressing RD3-Spot
RD3-KO Y-79 cells and RD3-SPOT-expressing cells were fixed by adding 27 µl of 37% formaldehyde (Wako) (final: 0.1%), incubated at RT for 10 min, quenched with 1 ml of 1 M Glycine-NaOH pH 7.5 (Nacalai) (final: 100 mM) at RT for 4 min, washed with 10 ml DPBS, and stocked at -80℃ as a cell pellet. For ...
ORGANISM(S): Homo Sapiens (human) 
2024-01-27 | PXD042933 | JPOST Repository
ProteomicsQuantification of oxidative modification of human serum albumin by MRM-HR
Two sampling areas were focused in this study; Tande tande hamlet as high-level natural background radiation area (HBRA) and Topoyo sub-district as normal level background radiation area (NBRA), which was done under coordination with Local Health Office. The former located in 2° 44’ 38.3’’ south lat...
ORGANISM(S): Homo Sapiens (human) 
2022-05-12 | PXD025947 | JPOST Repository
ProteomicsSerum proteome profiling of patients with non-small cell lung cancer treated with immune checkpoint blockade monotherapy
Shotgun proteomic profiling of serum from patients with non-small cell lung cancer (NSCLC) treated with immune checkpoint blockade (ICB) monotherapy. Top-14 abundant proteins were depleted (Pierce High-Select, Thermo Fisher Scientific), and tryptic digests were spiked with the Pierce Peptide Retenti...
ORGANISM(S): Homo Sapiens (human) 
2026-06-10 | PXD078247 | JPOST Repository
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