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HEK293T cells were transfected with SFB-tagged mTBK1 or an empty vector for 24 h, the cells were lysed with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM ethylenediaminetetraacetic acid) containing a complete protease inhibitor cocktail, followed by centrif...
ORGANISM(S): Homo Sapiens Mus Musculus 
2023-10-27 | PXD045961 |
To probe the binding sites of MeCbl on GSDME, 0.4 μM recombinant human GSDME (CSB-EP006766HU) purchased from CUSABIO (Wuhan, China) was incubated with 10 μM MeCbl in 100 μL buffer (20 mM HEPES, 150 mM NaCl and 50 μM DPA) at room temperature for 2 h and protected from light. 0.4 μM recombinant human ...
ORGANISM(S): Homo Sapiens 
2024-11-11 | PXD057768 |
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