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TranscriptomicsDifferentiated Truncation and Uridylation Patterns of miRNAs in Arabidopsis and Rice hen1 Mutants Suggest miRNAs May Re-program RISC
miRNA levels depend on both biogenesis and turnover. The methyltransferase HEN1 stabilizes plant miRNAs, animal piRNAs, and siRNAs in both kingdoms via 3' terminal methylation. Loss of HEN1 in plants results in non-templated oligo-uridylation and accelerated degradation of miRNAs. In hen1 mutants fr...
ORGANISM(S): Arabidopsis thaliana 
2013-07-22 | E-GEOD-35562 | biostudies-arrayexpress
TranscriptomicsHigh-throughput sequencing of small RNAs associated with Chalcone Synthase A (CHS-A) sense cosuppression in petunia
We report the application of sequencing-by-synthesis technology for high-throughput profiling of small RNAs involved in Chalcone synthase A (CHS-A) sense cosuppression in petunia. Sense cosuppression is a classical form of eukaryotic post-transcriptional gene silencing. It was first reported in tran...
ORGANISM(S): Petunia x hybrida 
2009-09-27 | E-GEOD-13764 | biostudies-arrayexpress
TranscriptomicsmiRNAs target conserved motifs of diverse genes encoding NB-LRRs to trigger trans-acting siRNAs in legumes.
Trans-acting siRNAs (tasiRNAs) negatively regulate target transcripts and are characterized by siRNAs spaced in 21-nucleotide 'phased' intervals. TasiRNAs have not been extensively described in many plant species. We identified dozens of new miRNAs in Medicago and soybean and confirmed 119 Medicago ...
ORGANISM(S): Arachis hypogaea 
2011-10-24 | E-GEOD-31061 | biostudies-arrayexpress
TranscriptomicsDCL4-Dependent Clusters of PhasiRNAs in Rice.
The osdcl4-1 mutant exhibits much severer developmental defects than dcl4 in Arabidopsis, suggesting that Os DCL4 may process broader substrates in rice. By deep sequencing of small RNAs from different tissues of wild types and osdcl4-1, we revealed that 21-nucleotide siRNAs were largely dependent o...
ORGANISM(S): Oryza sativa 
2012-03-29 | E-GEOD-22763 | biostudies-arrayexpress
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