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This project aims to analyze rice plasma membrane proteins related to resistance against rice blast infection. We extracted rice plasma membrane proteins before and after M.oryzae infection for 24h, and then used trypsin to digest and iTRAQ to label the peptides, HPLC-MS/MS was used to seperate and ...
ORGANISM(S): Oryza sativa (Rice) 
2022-05-19 | PXD028545 | Pride
A375 cells transduced with empty pLVX or pLVX-SPANXA-FLAG were subjected to immunoprecipitation using Flag beads. Elution from beads was performed by incubation with 3XFlag peptide for 1h at 4 C and eluates were subjected to tryptic digestion followed by LC-MS/MS, as described previously (PMID: 3099...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-24 | MSV000084617 | MassIVE
The study of protein-protein interactions is an essential process to understand the biological functions of proteins and the underlying mechanisms. Co-immunoprecipitation coupled with mass spectrometry (CoIP-MS) is one of the most extensively used high-throughput techniques to discover novel protein...
ORGANISM(S): Homo Sapiens 
2015-11-04 | MSV000079369 | MassIVE
Cells expressing Flag-tagged RNF5 or empty vector were incubated with the proteasome inhibitor MG132 to block protein degradation. Cell lysates were then prepared and immunoprecipitationed with anti-Flag antibody were performed, and eluates were digested with trypsin and analyzed on a Q Exactive.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-25 | MSV000083160 | MassIVE
This is the original mass spectrometry data, which includes 45 mass spectra data and a search database table. All these data and sample processing methods can refer to the ca in the manuscript
ORGANISM(S): Mus Musculus 
2021-02-19 | PXD024268 |
Sleep insufficiency is associated with various disorders (cardiovascular disease, neurodegenerative diseases, immunological disorders.) but the molecular bases for these associations are largely unknown. We used multiple omics techniques to examine changes in the blood of healthy male and female adu...
ORGANISM(S): Homo Sapiens 
2022-04-23 | PXD033402 |
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