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ProteomicsA fast, high-sensitivity 96-well plate based MICROFASP method for processing low microgram proteomics sample within 1.5 hours
We report a fast, high-sensitivity MICROFASP method that capable of completing sample preparation in less than 1.5 h which improved the throughput over 13 times compared to the previous reports. In this method, protein digestion time was dramatically shortened from 17 h to 20 min in the limited reac...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-05-07 | PXD053720 | Pride
Fast sample preparation  proteomics analysis  high-sensitivity  high-throughput 
ProteomicsSingle shot CZE-MS/MS for phosphoproteomics
The dependence of capillary zone electrophoresis separations on the charge state of analyte is useful for the analysis of many post-translational modifications in proteins. In this work, we coupled CZE to an Orbitrap Fusion Lumos Tribrid platform with advanced peak determination algorithm for phosph...
ORGANISM(S): Mus musculus (Mouse) 
2019-06-13 | PXD012888 | Pride
Cze Phosphoproteomics Orbitrap fusion lumos tribrid Advanced peak determination algorithm 
ProteomicsFilter aided, single tip (FAST) method for high throughput, ultrasensitive proteomics sample preparation reveals proteome asymmetry in Xenopus embryos
We report a filter aided, single tip (FAST) method for ultrasensitive proteomic sample preparation. To minimize sample loss and contamination, the method reduces the surface area of the filter to ~0.1 mm2, the total volume of reagents to < 10 μL, and the number of sample transfer steps to two. 25,88...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2022-07-12 | PXD015175 | Pride
Single blastomere Sample preparation Ultrasensitive proteomics 
ProteomicsIntegrated MICROFASP method with CZE based fractionation technique and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample
We report a loss-less two dimensional (2D) separation platform that integrated capillary zone electrophoresis (CZE) fractionation and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample. Protein digest was injected into the linear polyacrylamide coated capillary followed ...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD052735 | Pride
Submicrogram sample Two dimensional separation Capillary zone electrophoresis Microfasp method Proteome profiling 
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