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This study aimed to identify proteins interacting with Fha in Vibrio cholerae. A Strep-tagged Fha fusion protein (Fha-Strep) was expressed in Vibrio cholerae, and associated protein complexes were enriched using Strep-Tactin affinity purification following cell lysis. As a negative control, an sfGFP...
ORGANISM(S): Vibrio Cholerae O1 Biovar El Tor Str. N16961 
2026-05-01 | PXD078046 |

Ferroptosis is mediated by lipid peroxidation of phospholipids containing polyunsaturated fatty acyl moieties. Glutathione, the key cellular antioxidant capable of inhibiting lipid peroxidation via the activity of the enzyme glutathione peroxidase 4 (GPX-4), is generated directly from the sulfur-...

2023-01-11 | MTBLS5959 | MetaboLights

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
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