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TranscriptomicsAsthma III
Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data,...
ORGANISM(S): Homo sapiens 
2015-10-27 | E-GEOD-67940 | biostudies-arrayexpress
TranscriptomicsMapping of INS promoter interactions reveals its role in long-range regulation of SYT8 transcription
Insulin (INS) synthesis and secretion from pancreatic M-NM-2 cells are tightly regulated; their deregulation causes diabetes. Here we map INS-associated loci in human pancreatic islets by 4C and 3C techniques and show that the INS gene physically interacts with the SYT8 gene, located over 300 kb ...
ORGANISM(S): Homo sapiens 
2011-02-22 | E-GEOD-25862 | biostudies-arrayexpress
TranscriptomicsEffect of column cleanup on T7 based RNA linear amplification
Zhao et al. Amplification Table 4 This experiment was designed to evaluate the effect of column cleanup on the fidelity and yield of T7 based RNA linear amplification. BC2 total RNA was amplified using the Jeffrey lab protocol with or without G50 cleanup. Set of arrays organized by shared biological...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3562 | biostudies-arrayexpress
TranscriptomicsEffect of ligase on T7 based RNA linear amplification
Zhao et al. Amplification Table 3 This experiment was designed to evaluate the effect of ligase used in the second strand cDNA synthesis on the fidelity of T7 based RNA linear amplification. BC2 total RNA was amplified with or without ligase using Affymetrix protocol. Set of arrays organized by shar...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3561 | biostudies-arrayexpress
TranscriptomicsCorrelation between expression levels of different tumors measured by poly(A)+RNA and aRNA
Zhao et al. Amplification Table 7 This experiment was designed to determine the correlation between expression levels of defferent tumors (BC2 and BC91) measured by poly(A)+RNA and aRNA for each tumor. Total RNA was amplified using the Jeffrey lab protocol with G50 cleanup. Set of arrays organized b...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3563 | biostudies-arrayexpress
TranscriptomicsEffects of template switching (TS) primer and cDNA cleanup columns on T7 based RNA linear amplification
Zhao et al. Amplification Table 1 This experiment was designed to determine the effects of template switching (TS) primer and the type of columns used in ds cDNA cleanup on the fidelity of the T7 based RNA linear amplification. BC91 total RNA was amplified with or without TS primer and with two diff...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3560 | biostudies-arrayexpress
TranscriptomicsEffect of the amount of input total RNA on T7 amplification
Zhao et al. Amplification Table 9 This experiment was designed to evaluate the effect of the amount of input total RNA on the fidelity, reproducibility, and yield of T7 based RNA linear amplification. BC2 total RNA was amplified using the Jeffrey lab protocol. Different amounts of T7 primer were use...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3557 | biostudies-arrayexpress
TranscriptomicsAsthma II
Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data,...
ORGANISM(S): Homo sapiens 
2014-11-14 | E-GEOD-63142 | biostudies-arrayexpress
TranscriptomicsVariation in cDNA microarray analysis of gene expression using unamplified poly(A)+ RNA
Zhao et al. Amplification BC2 uM This experiment was designed to assess the reproducibility of microarray hybridization using poly(A)+RNA. BC2 total RNA was isolated from which poly(A)+RNA was subsequently isolated. Three arrays were hybridized on the same day using arrays from the same batch. Two w...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3559 | biostudies-arrayexpress
TranscriptomicsEffect of in vitro transcription time on the fidelity of T7-based RNA linear amplification
Zhao et al. Amplification Figure 1 This experiment was designed to evaluate the effect of in vitro transcription time on the fidelity, reproducibility, and yield of T7 based linear amplification. Duplicate reactions were performed at 37 degree for 2, 3, 4, 5, and 6 hours. Two additional 5-hour incub...
ORGANISM(S): Homo sapiens 
2005-11-03 | E-GEOD-3558 | biostudies-arrayexpress
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