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The HEK293T cells transiently expressing Flag-tagged empty vector or Flag-NRDE2-WT were lysed in NETN buffer (10 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40) and immunoprecipitated with anti-Flag M2 agarose (M8823; Sigma-Aldrich, MO, USA). After washing four times with the high stri...
ORGANISM(S): Homo Sapiens 
2024-03-31 | PXD051117 |
Proteomic analysis was performed using LC-MS/MS technology from Shanghai Aiao Biotechnology Co., Ltd.. The abundance of peptide fractions in the livers of Ptges2+/+ (WT) HFD and Ptges2-/- (KO) HFD groups was quantified using unlabeled quantitative methods and differentially expressed proteins in eac...
ORGANISM(S): Mus Musculus 
2022-07-19 | PXD035389 |
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