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TranscriptomicsProteomic and transcriptomic responses to thiamethoxam in the Sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies. All the B biotype whiteflies were maintained on cabbage. The susceptible strain (TH-S)...
ORGANISM(S): Bemisia tabaci 
2013-07-15 | E-GEOD-42102 | biostudies-arrayexpress
TranscriptomicsATAC-seq on endocardial cells from E12.5 mouse hearts in Tbx20 knockouts and wild type counterparts
We used ATAC-seq (Buenrostro et al, 2013) to identify regions of open chromatin in FACS sorted mouse endothelial cells from E12.5 hearts. Please note that the animals were injected at different dates, which resulted in different effects of the knockout, as indicated in the batch attribute of the s...
ORGANISM(S): Mus musculus 
2017-06-02 | E-MTAB-3972 | biostudies-arrayexpress
ProteomicsDifferential solubilization and top-down proteomics with FAIMS control experiments applied to mouse brain
5-35 mg brain tissue plugs from mouse left or right hemisphere were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homogenization buffer (H...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-12-25 | MSV000093727 | MassIVE
ProteomicsLarge scale, quantitative top-down proteomic analysis of human frontal gyrus brain tissue samples in the context of Alzheimer's disease
15-35 mg brain tissue plugs derived from dorsal lateral prefrontal cortex (DLPFC) were homogenized on ice with a pellet pestle homogenizer in a 1.5 mL LoBind Eppendorf tube after addition of 8 uL hexafluoroisopropanol (HFIP) / mg tissue. Once tissue was fully disrupted, an equivolume amount of homog...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-26 | MSV000093728 | MassIVE
TranscriptomicsLncRNAs expression profiles in the livers of 20-month-old male HBx transgenic mice and C57BL/6 wild-type mice
The hepatitis B virus X protein (HBx) has been implicated as an oncogene in both epigenetic modifications and genetic regulation during hepatocarcinogenesis, but the underlying mechanisms are not entirely clear. Long non-coding RNAs (lncRNAs), which regulate gene expression with little or no protein...
ORGANISM(S): Mus musculus 
2013-11-04 | E-GEOD-42185 | biostudies-arrayexpress
TranscriptomicsKetogulonigenium vulgare cells: co-culture mode vs. mono-culture mode
Transcriptional profiling of K. vulgare cells co-cultured with Bacillus megaterium compared to K. vulgare mono-cultured cells. Differentially expressed genes in co-cultured and mono-cultured K. vulgare cells were analyzed. The aim was to investigate the mechanisms of B. megaterium stimulating K. vul...
ORGANISM(S): Bacillus megaterium 
2012-04-25 | E-GEOD-37594 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of 100-day fetal skin revealing the differences of skin pigmentation in goats
We identified the mRNA and long non-coding RNA expression profiles of 100-day fetal skin between the dark and normal (white) skin in two breeds of goats using the deep RNA sequencing method. Case-control. Briefly, the 100-day fetal skin sampled from the normal and hyperpigmentated goats for deep seq...
ORGANISM(S): Capra hircus 
2016-06-11 | E-GEOD-69812 | biostudies-arrayexpress
TranscriptomicsExpression data from human hematopoietic stem and progenitor compartments from patients with acute myeloid leukemia with monosomy 7 and healthy controls
We applied a novel approach of parallel transcriptional analysis of multiple, highly fractionated stem and progenitor populations in a genetically defined subset of AML (AML with monosomy 7). We isolated phenotypic long-term HSC (LT-HSC), short-term HSC (ST-HSC), and committed granulocyte-monocyte p...
ORGANISM(S): Homo sapiens 
2012-09-05 | E-GEOD-35010 | biostudies-arrayexpress
TranscriptomicsDifferentiating human embryonic stem cells into corneal epithelial-like cells
Transplantation of ex vivo expanded limbal stem cells (LSC) is the main treatment for limbal stem cell deficiency though the clinical problem of donor tissues shortage. Recently, as the development of tissue engineering, embryonic stem cells (ESC) derived corneal epithelial-like cells (ESC-CEC) has ...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-70152 | biostudies-arrayexpress
TranscriptomicsExpression data from human hematopoietic stem and progenitor compartments from patients with acute myeloid leukemia with normal karyotype and healthy controls
We applied a novel approach of parallel transcriptional analysis of multiple, highly fractionated stem and progenitor populations from patients with acute myeloid leukemia (AML) and a normal karyotype. We isolated phenotypic long-term HSC (LT-HSC), short-term HSC (ST-HSC), and committed granulocyte-...
ORGANISM(S): Homo sapiens 
2012-09-05 | E-GEOD-35008 | biostudies-arrayexpress
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