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ProteomicsProfile the composition of reconstituted nuclear speckles
Nuclear extract from Hela Cells are incubated with recombinant SON IDR2 protein, resulting condensates are spun down and the compostion profiled by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2024-11-25 | PXD050371 | Pride
Nuclear speckles 
GenomicsCoactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude through REV-ERB Loading
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2015-12-07 | E-GEOD-67860 | biostudies-arrayexpress
GenomicsGenome-wide maps of BAF180 and REV-ERBα binding
We generated genome-wide cistromes of BAF180 subunit of the SWI-SNF chromatin remodeling complex in mouse liver at CT10 and CT22. In addition, we performed ChIP-Seq analysis on REV-ERBα in WT and SRC-2-/- mouse liver at CT10. We found circadian oscilation of BAF180 chromatin recruitment in mouse l...
ORGANISM(S): Mus musculus 
2015-12-07 | E-GEOD-67852 | biostudies-arrayexpress
GenomicsDetection of chromatin accessibility in WT and SRC-2-/- mice in liver
We futher characterized genome-wide chromatin accessibility of WT and SRC-2-/- mouse liver at CT10 through DNase-Seq. In addition,chromatin accessibility was significantly reduced in SRC-2-/- mouse liver compared to WT mice at CT10. DNase-Seq was carried out in WT and SRC-2-/- mice in liver at CT10...
ORGANISM(S): Mus musculus 
2015-12-07 | E-GEOD-67859 | biostudies-arrayexpress
TranscriptomicsGene expression of wild-type and Ppar-beta null primary keratinocytes, with and without infection with an activated Hras retrovirus, with and without the Ppar-beta specific ligand GW0742
Differential gene expression profiles were observed in response to Hras in either wild-type or Ppar-beta null primary keratinocytes and differentail gene edxpression profiles by GW0742 were only found in wild-type keratinocytes. Primary keratinocytes from wild-type and Ppar-beta null mice were eithe...
ORGANISM(S): Mus musculus 
2012-04-11 | E-GEOD-32498 | biostudies-arrayexpress
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