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Nano-flow LC-MS/MS analysis of 293T digest in two gradients.
ORGANISM(S): Homo Sapiens 
10 μg 293T was spiked with different amounts ( 0.2, 0.3, 0.4, 0.5 μg) of E.coli. Performed the ablation analysis to evaluate the DNN model in DeepRTAlign. Compared DeepRTAlign with Quandenser. Compared DeepRTAlign with MaxQuant’s MBR and FragPipe’s MBR. Generalizability evaluation on simulated datas...
ORGANISM(S): Homo Sapiens Escherichia Coli 
PRM datasets and Skyline results for the validation of ion-centric findings.
ORGANISM(S): Homo Sapiens 
Four samples were prepared: 293T digest (73%, 91%, 97% and 99%) offset by varied proportions (27%, 9%, 3% and 1%) of E.coli digest. 10 μg of 293T and E. coli mixtures were loaded each time and the DDA data were collected from six Orbitrap Exploris 480 mass spectrometers with the same settings.
ORGANISM(S): Homo Sapiens 
Micro-flow LC-MS/MS analysis of pure 293T or E.coli.
ORGANISM(S): Homo Sapiens 
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