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TranscriptomicsUsing next generation sequencing to study the genetic diversity of candidate live attenuated Zika vaccines
Zika virus (ZIKV) is a mosquito-transmitted positive-sense RNA virus in the family Flaviviridae. Candidate live-attenuated vaccine (LAV) viruses with engineered deletions in the 3’UTR provide immunity and protection in animal models of ZIKV infection, and phenotypic studies show that LAVs retain pro...
ORGANISM(S): Zika virus 
2020-04-13 | E-MTAB-8905 | biostudies-arrayexpress
MetabolomicsGlycerophospholipid remodeling is critical for orthoflavivirus infection

Flavivirus infection is tightly connected to host lipid metabolism. Here, we performed shotgun lipidomics of cells infected with neurotropic Zika, West Nile and tick-borne encephalitis viruses, as well as dengue and yellow fever virus. Early in infection specific lipids accumulated, e.g., neutral...

2025-02-24 | MTBLS11030 | MetaboLights
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV) strain, PE243
Vero cells were infected with the Asian/American Zika virus (ZIKV) strain, PE243, at MOI:3 to assess the viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was carried out, following which ribosome...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12971 | biostudies-arrayexpress
TranscriptomicsRNASeq of African green monkey (Vero) cells and human malignant glioblastoma multiforme (U251) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV), PE243; and the African ZIKV strain, Dak84
Vero and U251 cells were infected with the Asian/American ZIKV (PE243) and the African ZIKV (Dak84) at MOI:3 to assess the viral transcriptome in two cell lines. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. Ribosomal RNA was remov...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12967 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells and human malignant glioblastoma multiforme (U251) cells infected at a multiplicity of infection (MOI) of 3 with the Asian/American Zika virus (ZIKV), PE243; and the African ZIKV strain, Dak84
Vero and U251 cells were infected with the Asian/American ZIKV (PE243) and the African ZIKV (Dak84) at MOI:3 to assess the viral transcriptome in two cell lines. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide pre-treatment. RNase I treatment was ...
ORGANISM(S): Homo sapiens 
2024-09-04 | E-MTAB-12970 | biostudies-arrayexpress
TranscriptomicsRNASeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12968 | biostudies-arrayexpress
TranscriptomicsRiboSeq of African green monkey (Vero) cells infected at a multiplicity of infection (MOI) of 3 with different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics
Vero cells were infected with the different Zika virus (ZIKV) mutants (American WT, African-like, uORF1-KO and uORF2-PTC1) generated by reverse genetics at MOI:3 to assess their viral transcriptome. Samples were harvested at 24 hours post-infection (h p.i.) by flash-freezing, without cycloheximide p...
ORGANISM(S): Chlorocebus sabaeus 
2024-09-04 | E-MTAB-12969 | biostudies-arrayexpress
ProteomicsQuantitative proteomic analysis of ZIKV infected C6/36 cells
An iTRAQ-based quantitative proteomic analysis of ZIKV infected Aedes albopictus C6/36 cells was performed to investigate host proteins involved in ZIKV infection process. A total of 3,544 host proteins were quantified, with 200 being differentially regulated. Bioinformatics analysis on regulated ho...
ORGANISM(S): Aedes albopictus 
2017-04-21 | PXD004727 | Pride
Zikv C6/36 Itraq 
ProteomicsMass spectrometry analysis of Extracellular vesicles derived from naive HUVEc cells or ZIKV-infected HUVEc cells
In this project, we aimed to explore the role of extracellular vesicles (EVs) in Zika virus (ZIKV) infection. We preferred antibody capture to ltracentrifugation for exosome isolation. The EVs derived from naive HUVEc cells or ZIKV-infected HUVEc cells were harvested and analyzed by mass spectrometr...
ORGANISM(S): Homo sapiens (Human) 
2023-01-19 | PXD039152 | Pride
Mass specctrometry Extracellular vesicles Zikv infection 
ProteomicsParacrine IFN response limits ZIKV infection in human Sertoli cells
Zika virus (ZIKV) is unique among mosquito-borne flaviviruses in its ability to be sexually transmitted. The testes have been implicated as sites of long-term ZIKV replication, and our previous studies have identified Sertoli cells (SC), the nurse cells of the seminiferous epithelium that govern spe...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD025133 | Pride
Sartoli cells Interferon Zika virus 
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