Proteomics,Multiomics

Dataset Information

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The MPK8-TCP14 pathway promotes seed germination in Arabidopsis

ABSTRACT: Accurate control of dormancy release and germination is critical for successful plantlet establishment. In this study, we characterized mutants of Arabidopsis MAP kinase 8 (MPK8) presenting altered dormancy. We identified Teosinte Branched1/Cycloidea/Proliferating cell factor 14 (TCP14), a key transcription factor for germination, as a partner of MPK8 and investigated the processes controlled by MPK8-TCP14 pathway. Kinase assay was performed using immunoprecipitated MPK8-HA protein and recombinant GST-TCP14 protein as target to find TCP14 MPK8-specific phosphorylation sites.

OTHER RELATED OMICS DATASETS IN: MODEL1109130000MODEL1311110000MODEL1909260003MODEL1311110001MODEL1909260004MODEL1703310000MODEL1909260005MODEL1909260006

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Cédric Pionneau  

LAB HEAD: Solenne Chardonnet

PROVIDER: PXD013544 | Pride | 2019-07-25

REPOSITORIES: Pride

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Publications

The accurate control of dormancy release and germination is critical for successful plantlet establishment. Investigations in cereals hypothesized a crucial role for specific MAP kinase (MPK) pathways in promoting dormancy release, although the identity of the MPK involved and the downstream events remain unclear. In this work, we characterized mutants for Arabidopsis thaliana MAP kinase 8 (MPK8). Mpk8 seeds presented a deeper dormancy than wild-type (WT) at harvest that was less efficiently all  ...[more]

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