Browse
Submit Data
Databases
API
Help

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-19686

ABSTRACT: shGFP- and shQK-transduced human Hs683 cells

PROVIDER: E-GEOD-19686 | ExpressionAtlas | 2015-08-07

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Similar Datasets

Homo sapiens

Project description:shGFP- and shQK-transduced human Hs683 cells

| PRJNA123977 | ENA

Homo sapiens

Project description:Leukemia fusion-gene transduced human cord blood cells

| PRJNA98353 | ENA

Compare miRNA expression profiles of human iNSC, H9-NSC, fibroblast

Project description:miRNA profiling of human H9-derived neural stem cells (H9-NSCs) comparing control human adult dermal fibroblasts (hDFs), SOX2-transduced human induced neural stem cells (hDF-iNSC (SOX2)), SOX2/HMGA2-transduced human induced neural stem cells (hDF-iNSC (SOX2/HMGA2)). Goal was to determine the global miRNA expression between the groups.

2014-07-11 | GSE59301 | GEO

RNA-seq following TBL1XR1 KD in human CD34+CD38- cord blood cells

Project description:We performed RNA-seq on CD34+CD38- human cord blood (CB) cells transduced with sh_Control or sh_TBL1XR1 lentiviruses to identify differentially expressed genes. Sorted CD34+CD38- cells from 3 independent cord blood pools were transduced with lentiviruses containing BFP reporter gene. BFP+ cells were sorted 3 days post-transduction and RNA was harvested for library preparation and sequencing.

| EGAS00001005869 | EGA

RNA-seq following miR-130a overexpression (OE) in human CD34+cord blood cells

Project description:We performed RNA-seq on CD34+ human cord blood (CB) cells transduced with Control or miR-130a OE lentiviruses to identify differentially expressed genes. Sorted CD34+ cells from 3 independent cord blood pools were transduced with lentiviruses containing mOrange (mO) reporter gene. mO+ cells were sorted 3 days post-transduction and RNA was harvested for library preparation and sequencing.

| EGAS00001005868 | EGA

Metabolomics Workbench ST000819 - GNPS - Looking for changes in lipid levels with knock out of ACADM (shACADM) (part II)

Project description:GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.

2018-08-02 | MSV000082739 | GNPS

A dominant-negative isoform of IKAROS expands primitive normal human hematopoietic cells

Project description:Human cord blood CD34+ cells transduced with control of IK6 lentivirus were transplanted into NSG mice. Control and IK6 transduced CD34+38- progeny were acquired 10 weeks later by FACS

2014-09-14 | GSE60957 | GEO

Characterising early phosphorylation events upon HER2 protein overexpression

Project description:HER2 transduced cells which we will refer to as HER2-DOX –. These cells are 99% GFP positive (i.e., 99% cells have HER2 transduced, un-induced). As a control, we had GFP empty vector transduced MCF10A cells (95% have GFP transduced). Both cell types in triplicates. We had 4 time-points 0h, 30 mins, 4hours, and 7 hours (time duration for which HER2 will be induced). DOX was added to the GFP-MCF10A cells as a control. Only 1ug/ml of DOX was be used.

2023-01-23 | PXD034105 | Pride

YAP conveys an invasive phenotype to clear cell renal cell carcinoma cell lines via endothelin-2

Project description:To determine the biological mechanisms underlying the oncogenic properties of YAP in ccRCC the human ccRCC cell line MZ1774 was transduced with lentivirus containing a shRNA-cassette targeting YAP-mRNA. Expression profiles of MZ1774 YAP knockdown cells were compared to mock-transduced control cells.

2014-12-23 | GSE50053 | GEO

Mus musculus

Project description:Gene expression profiles of the Cited4-siRNA-transduced cells and the non-transduced cells.

| PRJNA268180 | ENA