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MiR-148b modulates the expression of multiple genes

ABSTRACT: To identify which miR-148b targets were involved in tumorigenesis, a microarray analysis was performed for miR-148b over-expressing cells versus controls and 129 (49 up and 80 down) modulated genes were revealed. The effects of miR-148b on cancer progression depend on the direct and indirect regulation of multiple target genes. To identify miR-148b modulated genes, MDA-MB-231 cells were transfected with miR-148b precursors or negative controls (pre-miR-148b or control) and used 48h later for microarray and western blot (WB) analyses. When a “Whole Human Genome Oligo Microarray” (Agilent) platform was employed, 129 differentially expressed genes (49 upregulated, 80 downmodulated) were found at 48h, considering a fold change (FC) cut of 1.5 and a false discovery rate (FDR) of 16% (Table S4). Crossing these results with the list of putative miR-148b targets (3642) obtained by the miRecords System, we observed that 33 of the modulated genes were also miR-148b predicted targets and interestingly, 26 out of these 33 genes were downmodulated.

OTHER RELATED OMICS DATASETS IN: PRJNA142187PRJNA142189PRJNA136411

PROVIDER: E-GEOD-26662 | ExpressionAtlas | 2015-08-12

REPOSITORIES: ExpressionAtlas

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miR148b is a major coordinator of breast cancer progression in a relapse-associated microRNA signature by targeting ITGA5, ROCK1, PIK3CA, NRAS, and CSF1.

Cimino Daniela D   De Pittà Cristiano C   Orso Francesca F   Zampini Matteo M   Casara Silvia S   Penna Elisa E   Quaglino Elena E   Forni Marco M   Damasco Christian C   Pinatel Eva E   Ponzone Riccardo R   Romualdi Chiara C   Brisken Cathrin C   De Bortoli Michele M   Biglia Nicoletta N   Provero Paolo P   Lanfranchi Gerolamo G   Taverna Daniela D  

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20121211 3

Breast cancer is often fatal during its metastatic dissemination. To unravel the role of microRNAs (miRs) during malignancy, we analyzed miR expression in 77 primary breast carcinomas and identified 16 relapse-associated miRs that correlate with survival and/or distinguish tumor subtypes in different datasets. Among them, miR-148b, down-regulated in aggressive breast tumors, was found to be a major coordinator of malignancy. In fact, it is able to oppose various steps of tumor progression when o  ...[more]

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