ABSTRACT: Transcriptional profiling of Zea mays roots infected by necrotrophic pathogen Phytophthora cinnamomi for 6 or 24 hours against mock-infected controls
Project description:Maize (Zea mays L.) was hydroponically grown for 14 days and then stressed with hypoxia. Maize roots were sampled after 24 hours and analyzed by mass spectrometry.
Project description:In the present study we have assessed, by transcriptional profiling, the systemic defense response of Zea mays plants to the ear rotting pathogen Fusarium verticilioides induced by the beneficial fungus Trichoderma atroviride
Project description:We developed a method to synchronize the induction of lateral roots in primary and adventitious roots of Zea mays, and used it to perform a genome-wide transcriptome analysis of the pericycle cells in front of the phloem poles during lateral root initiation.
Project description:In this study we perform a transcriptomics analysis of two maize (Zea mays) organs, roots and leaves, from plants grown in the presence of a sufficient (1000 uM) or limiting (10 uM) concentration of soil phosphate.
Project description:Proteomic data from dengue virus infected U-937 cells. PVD_C samples were infected via antibody-mediated or DC-SIGN receptor mediated entry routes with wild-type DENV-4 or mock inoculum; time points 2, 8, 16, and 24 hours; 5 biological replicates. PVD_L samples were infected via antibody-mediated or DC-SIGN receptor mediated entry routes with wild-type DENV-1 or mock inoculum; time points 2, 6, 10, 18, 24, and 30 hours; 5 biological replicates.
Project description:Infection of RAW264.7 cells with RHΔku80 parasites or mock-infection for 24 hours To measure changes in gene expression induced in macropahges upon Toxoplasma infection, we infected RAW 264.7 macrophages in cell culture with RHΔku80 parasites or syringe-lysed human foreskin fibroblast monolayers (mock-infected). RNA was harvested 24 hours post infection.
Project description:Zea mays transcriptome profiling of infected seedlings by the Ustilago maydis wildtype and the seedling specific effector mutant demonstrated the variation of gene expression in the mutant and the classes of genes that are absent in the mutant as compared to the wildtype U. maydis SG200 strain. Two dye competitive hybridizations were performed on Agilent Oligo arrays. Comparison were done 1) with mock and infected samples at 6dpi. 2) between the two 6dpi infected samples with wildtype and the secreted effector mutant
Project description:We developed a method to synchronize the induction of lateral roots in primary and adventitious roots of Zea mays, and used it to perform a genome-wide transcriptome analysis of the pericycle cells in front of the phloem poles during lateral root initiation. Lateral roots were induced in primary and adventitious roots of Maize. For the primary root, plants were germinated and grown 64 hours in NPA 50 µM, and then transfered to NAA 50 µM. For the adventitious roots, plants were germinated and grown in water for 6 days, then tranfered 4 days in NPA 25 µM, and finally transfered to NAA 25 µM. For all these roots, pericycle cells located in front of the phloem poles in segments of roots located between 5 and 10 mm distance from the root tip were isolated using laser capture microdissection after cryosection. Material was sampled at 0 hours (NPA) and after 2, 3 and 4 hours of NAA treatment, for both the primary and adventitious roots and also after 6 hours and 9 hours of NAA treatment for the adventitious roots.
Project description:Purpose: to compare host gene expression in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze viral chromatin accessibility