Genomics,Multiomics

Dataset Information

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Gene expression profile of adult male offspring from ethanol-consuming mice


ABSTRACT: Moderate alcohol consumption during pregnancy can result in a heterogeneous range of neurobehavioural and cognitive effects, termed fetal alcohol spectrum disorders (FASD). We have developed a mouse moder of FASD that involves moderate ethanol exposure throughout gestation achieved by voluntary maternal consumption. This model results in phenotypes relevant to FASD. Since ethanol is known to directly affect the expression of genes in the developing brain leading to abnormal cell death, changes to cell proliferation, migration, and differentiation, and potential changes to epigenetic patterning, we hypothesize that this leaves a long-term footprint on the adult brain. However, the long-term effects of prenatal ethanol exposure on brain gene expression, when behavioural phenotypes are apparent, are unclear. We used two independent microarray experiments and focused on the genes identified by both to evaluate the genome-wide alterations to the adult brain transcriptome caused by prenatal ethanol exposure via moderate maternal drinking. To generate samples, two independent groups of female C57BL/6J mice were given access to 10% ethanol in water or water only. Control females had access to water only. Females were mated and continued to drink from gestational day 0 to pup postnatal day 10. Whole brain RNA from adult (postnatal day 70) male ethanol-exposed offspring was extracted. For experiment 1, RNA samples from three mice were pooled to reduce litter effects and the pooled samples were hybridized on Affymetrix arrays (2 control and 2 ethanol chips, total n=12 mice). For experiment 2, RNA from two mice were pooled per chip and three arrays per treatment were used (3 control, 3 ethanol, total n=12 mice).

REANALYSIS of: E-GEOD-34305

OTHER RELATED OMICS DATASETS IN: PRJNA151517

ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-34305 | ExpressionAtlas | 2015-09-22

REPOSITORIES: ExpressionAtlas

Dataset's files

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Action DRS
E-GEOD-34305-analysis-methods.tsv Tabular
E-GEOD-34305-atlasExperimentSummary.Rdata Rdata
E-GEOD-34305-configuration.xml Xml
E-GEOD-34305-percentile-ranks.tsv Tabular
E-GEOD-34305.condensed-sdrf.tsv Tabular
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Publications

Long-term alterations to the brain transcriptome in a maternal voluntary consumption model of fetal alcohol spectrum disorders.

Kleiber Morgan L ML   Laufer Benjamin I BI   Wright Elise E   Diehl Eric J EJ   Singh Shiva M SM  

Brain research 20120417


Many women continue to consume low to moderate quantities of alcohol during pregnancy, which can result in the variable neurobehavioural effects in the absence of physiological abnormalities that characterize fetal alcohol spectrum disorders (FASD). Previously, we reported that a mouse model for FASD based on voluntary maternal ethanol consumption throughout gestation resulted in offspring that showed mild developmental delay, anxiety-related traits, and deficits in spatial learning. Here, we ex  ...[more]

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