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E-GEOD-37286

ABSTRACT: Drug tolerance development of mouse Bcr/Abl pre-B ALL cells on irradiated MEFs

OTHER RELATED OMICS DATASETS IN: PRJNA159405

PROVIDER: E-GEOD-37286 | ExpressionAtlas | 2015-09-28

REPOSITORIES: ExpressionAtlas

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Mus musculus

Project description:Drug tolerance development of mouse Bcr/Abl pre-B ALL cells on irradiated MEFs

| PRJNA159405 | ENA

Expression in irradiated MEFs exposed to murine acute lymphoblastic leukemia cells

Project description:Primary pre-B acute lymphoblastic (ALL) cells do not proliferate long-term ex vivo without the presence of stromal support. We developed and use an ex vivo co-culture model, consisting of mouse leukemic pre-B Bcr/Abl-expressing ALL cells grown with mitotically inactivated mouse embryonic fibroblasts (MEFs). This system provides a generic type of environmentally-mediated protection to the ALL cells, because when the ALL cells are treated with a moderate dose of a therapeutic drug, drug-resistant ALL cells can be recovered after a 1-2 week period of culture. Some of the factors produced by stromal cells that provide protection to ALL cells have been identified. However, it is unclear if the presence of drug-treated ALL cells affects the stromal fibroblasts. The current study was initiated to examine this using expression profiling on the irradiated MEFs. We isolated RNA from irradiated wild-type MEFs after a 9-day culture period. MEFs had either been exposed to DMSO, to nilotinib, a drug that is not expected to affect them, or to ALL cells treated with DMSO or nilotinib. MEFs and ALL cells were physically separated by a Transwell membrane of a pore size that did not allow the ALL cells to migrate towards the MEFs, but allowed the exchange of diffusible molecules.

2011-12-01 | E-GEOD-33329 | biostudies-arrayexpress

Expression in irradiated MEFs exposed to murine acute lymphoblastic leukemia cells

2011-12-01 | GSE33329 | GEO

Microarray analysis of gene expression in ATM-deficient MEFs

Project description:The expression of interferon-related genes was more enhanced in irradiated ATM-deficient mouse embryonic fibroblasts (MEFs) than in irradiated ATM wild-type MEFs.

2012-06-20 | GSE23116 | GEO

PP2A mediates malignant pre-B cell survival through Lineage-Specific control of cellular redox homeostasis

Project description:Ppp2r1afl/fl mouse bone marrow pre-B cells were transduced with an BCR-ABL1 vector. The BCR-ABL1 transduced Ppp2r1afl/fl pre-B cells were then transduced with an empty vector (EV), or a Cre vector for Cre-mediated PP2A deletion. Effect of PP2A deletion in the BCR-ABL1 pre-B cells were studied by Affymetrix GeneChip Mouse Genome ST1.0 Array

2018-02-12 | GSE83742 | GEO

Microarray analysis of gene expression in ATM-deficient MEFs

Project description:The expression of interferon-related genes was more enhanced in irradiated ATM-deficient mouse embryonic fibroblasts (MEFs) than in irradiated ATM wild-type MEFs. Nonirradiated-ATM-WT vs Irradiated-ATM-WT vs Nonirradaited-ATM-KO vs IrradiatedATM-KO

2012-06-19 | E-GEOD-23116 | biostudies-arrayexpress

Microarray analysis of gene expression in mouse embryonic fibroblasts (MEFs) by addition of serum from L/MDR irradiated mice at 10 and 20 days.

Project description:The expression changes were detected by addition of serum form L/MDR irradiated mice at 10 and 20 days in mouse embryonic fibroblasts (MEFs). (Expression of MEFs by addition of serum from nonirradiated mice) vs (Expression of MEFs by addition from L/MDR irradiated mice)

2013-12-31 | E-GEOD-37958 | biostudies-arrayexpress

Microarray analysis of gene expression in mouse embryonic fibroblasts (MEFs) by addition of serum from L/MDR irradiated mice at 10 and 20 days.

Project description:The expression changes were detected by addition of serum form L/MDR irradiated mice at 10 and 20 days in mouse embryonic fibroblasts (MEFs).

2013-12-31 | GSE37958 | GEO

A mechanistic rationale for targeting the unfolded protein response in pre-B acute lymphoblastic leukemia [array]

Project description:The plasma cell transcription factor XBP1 is critical for terminal differentiation of B cells into plasma cells but has no known role at earlier stages of B-cell development. Here we show that XBP1 is not only important during early B-cell development and for survival of pre-B cells but also protects pre-B ALL cells. Among pre-B ALL subset, XBP1 was hypomethylated and highest expressed in the Ph+ ALL subset. Cre-mediated deletion of XBP1 in a mouse model of Ph+ ALL compromised proliferation and viability and prolonged survival of leukemia-bearing mice. Interestingly, XBP1 expression levels were positively transcriptionally regulated by STAT5 and negatively by BACH2 and BCL6. High XBP1 expression in high risk ALL patients at the time of diagnosis predicted poor outcome in two clinical trials. Clinically, small-molecule inhibition of IRE1-dependent XBP1-activation caused cell death of patient-derived pre-B ALL cells and affected leukemia-initiation in transplant recipient mice. Collectively, these studies identify XBP1 as an important survival factor and as a potential therapeutic target to overcome drug-resistance in pre-B ALL. Genome-wide profiling of mRNA levels in BCR-ABL1 transduced murine Grp78 fl/fl pre-B cells with an empty vector (Grp78 wild type, MIG-1,2,3) and Cre (Grp78 deletion, CRE-1,2,3).

2014-04-24 | E-GEOD-53684 | biostudies-arrayexpress

CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis

Project description:In B lymphopoiesis, activation of the pre-B cell antigen receptor (pre-BCR) is associated with both cell cycle exit and Igk recombination. Yet, how the pre-BCR mediates these functions remains unclear. Herein, we demonstrate that the pre-BCR initiates a feed-forward IRF4-CXC Receptor 4 (CXCR4) amplification loop. ERK activation by CXCR4 then directs the development of small and immature B cells including orchestrating cell cycle exit, pre-BCR repression, Igk recombination and BCR expression. In contrast, escape from IL-7 and pre-BCR expression have only modest effects on B cell developmental transcriptional and epigenetic programs. These data demonstrate a direct and central role for CXCR4 in orchestrating late B cell lymphopoiesis. Furthermore, in the context of previous findings, our data provide a three-receptor system sufficient to recapitulate the essential features of B lymphopoiesis in vitro.

2019-06-18 | GSE129311 | GEO

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