Transcriptomics,Multiomics

Dataset Information

15

Transcription profiling by array of Caenorhabditis elegans mutant for prg-1 and prg-2


ABSTRACT: piRNAs are required to maintain germline integrity and fertility but their mechanism of action is poorly understood. Here we demonstrate that C. elegans piRNAs silence transcripts in trans through imperfectly complementary sites. We find that target silencing is independent of Piwi endonuclease activity or “slicing”. Instead, we show that piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene, pseudogene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes but not pseudogenes or transposons. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNAi in C. elegans. Affymetrix mRNA expression data from wild-type and two independent prg-1;prg-2 double mutant C. elegans strains (mRNA)

REANALYSIS of: E-GEOD-37432

ORGANISM(S): Caenorhabditis elegans  

PROVIDER: E-GEOD-37432 | ExpressionAtlas | 2014-08-15

REPOSITORIES: ExpressionAtlas

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Publications

Function, targets, and evolution of Caenorhabditis elegans piRNAs.

Bagijn Marloes P MP   Goldstein Leonard D LD   Sapetschnig Alexandra A   Weick Eva-Maria EM   Bouasker Samir S   Lehrbach Nicolas J NJ   Simard Martin J MJ   Miska Eric A EA  

Science (New York, N.Y.) 20120614 6094


Piwi-interacting RNAs (piRNAs) are small RNAs required to maintain germline integrity and fertility, but their mechanism of action is poorly understood. Here we demonstrate that Caenorhabditis elegans piRNAs silence transcripts in trans through imperfectly complementary sites. Target silencing is independent of Piwi endonuclease activity or "slicing." Instead, piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene and transpos  ...[more]

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