Transcriptomics

Dataset Information

122

Activating CD8 T cell clones via the T cell receptor complex in the absence and presence of cyclosporine A


ABSTRACT: Comparison of a cyclosporine-resistant murine CD8 T cell clone to a cyclosporine-sensitive murine T cell clone during activation (CSA = Cyclosporine A) Chronic allograft rejection is the leading cause of morbidity/mortality in solid organ and bone marrow transplant patients. It occurs in the setting of potent calcineurin and mTOR inhibitor therapies, implying that T cells mediating chronic rejection can function with compromised calcineurin-NFAT or IL-2 receptor signaling pathways. In murine models the T cells mediating allograft rejection in the setting of calcineurin inhibitor therapy are CD8 T cells making IFN-gamma without a requirement for perforin. It is not known whether these pathologic T cells are a unique subset or how they function. In parallel other animal model research has shown that alloepthelial cells residing in the donor organ are critical targets for rejection. While investigating T cell-alloepithelial cell interactions, we discovered an unusual alloreactive CD8 T cell population that recognized MHC class II I-Abm12. A CD8 T cell clone derived from that population was intrinsically-resistant to cyclosporine and rapamycin without prior exposure to either in vivo or in vitro. Microarray analysis comparing the novel CD8 T cell clone to a conventional CD8 CTL clone specific for MHC class I Kbm1 suggested that the TCR signaling pathway responsible for cyclosporine-resistance utilized the Aryl Hydrocarbon Receptor (AHR). Two T cell clones x 2 experimental conditions x 4 replicates

REANALYSIS of: E-GEOD-46122

ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-46122 | ExpressionAtlas | 2015-07-13

REPOSITORIES: ExpressionAtlas

Similar Datasets

2014-04-14 | E-GEOD-46122 | ArrayExpress
2018-10-26 | PXD005765 | Pride
2008-06-14 | E-GEOD-6259 | ArrayExpress
2015-10-22 | E-MEXP-2000 | ExpressionAtlas
2006-12-01 | GSE6259 | GEO
2010-01-08 | E-MEXP-2000 | ArrayExpress
2019-05-20 | PXD013066 | Pride
2014-02-28 | E-GEOD-49063 | ArrayExpress
2011-08-09 | E-GEOD-31082 | ArrayExpress
2014-06-01 | E-GEOD-47621 | ArrayExpress