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E-GEOD-4695

ABSTRACT: Transcription profiling of mouse dermal fibroblasts following exposure to endothelin-1

OTHER RELATED OMICS DATASETS IN: PRJNA96743PRJNA96745

PROVIDER: E-GEOD-4695 | ExpressionAtlas | 2015-10-12

REPOSITORIES: ExpressionAtlas

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Transcription profiling of mouse primary dermal fibroblasts treated with endothelin-1

Project description:Mouse primary dermal fibroblasts were treated with 100 nM endothelin-1 (ET1) synthetic peptide for 24 hours. Control samples received no ET1 peptide. The experiment compared treated to untreated to identify gene expression changes due to ET1 exposure. There are three biological replicates for both control and treated samples. These biological replicates represent separate derivations of primary dermal fibroblasts from genetically identical mouse litters aged 0-3 days.

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Transcription profiling of mouse dermal fibroblasts following exposure to endothelin-1

Project description:To determine if aberrant activation of endothelin-1 (Et1) could lead to the dysregulation of many downstream genes, we exposed fibroblasts to exogenous ET1 peptide and assayed for transcriptional changes by microarray. Mouse dermal fibroblasts were treated with exogenous Et1 peptide for 24 hours. ET1 treatment resulted in significant expression changes - primarily downregulation - of a number of genes. In particular, Tgf-beta-2 and Tgf-beta-3 were among the downregulated genes, which in turn alter the expression status of their many target genes. These data suggest that the stable silencing of Et1 is important for the phenotypic stability of dermal fibroblasts, and perhaps many other cell types as well.<br><br>Three separate biological replicates were derived for both control and treated samples. The primary dermal fibroblasts were derived by explant procedure from the skin of mouse pups aged 0-3 days. By passage 5, cells were split to two separate cultures-- one with 100nM synthetic Et1 peptide added to the medium (treated) and the other with nothing added (control). Cells were exposed to Et1 for 24 hrs, then treated and control populations were harvested for total RNA.

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Project description:Transcription profiling by array of mouse primary dermal fibroblasts after treatment with endothelin-1

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