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Omics score: 0

E-GEOD-56678

ABSTRACT: Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

OTHER RELATED OMICS DATASETS IN: PRJNA244297

PROVIDER: E-GEOD-56678 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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Dataset's files

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			Action	DRS
	E-GEOD-56678-analysis-methods.tsv	Tabular
	E-GEOD-56678-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-56678-configuration.xml	Xml
	E-GEOD-56678-configuration.xml.beforeQC	Xml
	E-GEOD-56678-percentile-ranks.tsv	Tabular

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Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

Project description:Calu-3 2B-4 cells were stimulated with interleukin-1a or TNFa. Transcriptional analysis of the extracted RNA was done by microarray.

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Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

Project description:Calu-3 2B-4 cells were stimulated with interleukin-1a or TNFa. Transcriptional analysis of the extracted RNA was done by microarray. Calu-3 2B-4 cells were washed with phosphate-buffered saline (PBS) and treated with either IL-1a (0.001ng/ml) or TNFa (0.05ng/ml) or mock diluted in PBS for 40 min at 37C. Following treatment, cells were washed 3 times, and fresh medium was added. Triplicate Calu-3 2B4 cultures and triplicate time-matched mock-infected controls were harvested at 3, 6 and 24h for IL-1a and 6 and 24h for TNF post-exposure for transcriptional analysis.

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