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Omics score: 0

Homo sapiens

ABSTRACT: Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

OTHER RELATED OMICS DATASETS IN: E-GEOD-56678

PROVIDER: PRJNA244297 | ENA |

REPOSITORIES: ENA

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E-GEOD-56678

Project description:Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

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Project description:Calu-3 2B-4 cells were stimulated with interleukin-1a or TNFa. Transcriptional analysis of the extracted RNA was done by microarray.

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Transcriptional analysis of Calu-3 2B-4 cells stimulated with Interleukin-1a and TNFa.

Project description:Calu-3 2B-4 cells were stimulated with interleukin-1a or TNFa. Transcriptional analysis of the extracted RNA was done by microarray. Calu-3 2B-4 cells were washed with phosphate-buffered saline (PBS) and treated with either IL-1a (0.001ng/ml) or TNFa (0.05ng/ml) or mock diluted in PBS for 40 min at 37C. Following treatment, cells were washed 3 times, and fresh medium was added. Triplicate Calu-3 2B4 cultures and triplicate time-matched mock-infected controls were harvested at 3, 6 and 24h for IL-1a and 6 and 24h for TNF post-exposure for transcriptional analysis.

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