E-GEOD-58082
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA248877PRJNA248875PRJNA248878
PROVIDER: E-GEOD-58082 | ExpressionAtlas | 2016-05-03
REPOSITORIES: ExpressionAtlas
Similar Datasets
Project description:We investigated the heat-dependent in-vivo interactome of Arabidopsis thaliana SKD1 using a transgenic 35S::GFP-SKD1 line. Potential interactors of GFP-SKD1 were co-immunoprecipitated from cell extracts of untreated or heat-treated rosette leaves and analyzed by mass spectrometry. A line overexpressing free YFP (35S::YFP) was used as a negative control. For each genotype and condition, proteins of three biological replicates were analyzed. An in-solution digest was performed on the beads, and peptides were subjected to liquid chromatography and tandem mass spectrometry (LC-MS/MS, Dr. S. Müller, CECAD/CMMC Proteomics Facility Cologne).
2021-06-23 | PXD025028 | Pride
Project description:To identify possible mitochondrial DNA binding targets of SHOT1/MTERF18 (AT3G60400), C-terminal GFP fusion constructs under either a constitutive 35S promoter (35S::SHOT1-GFP) or the SHOT1 native promoter (SHOT1p::SHOT1-GFP) were made. The transgenes were introduced into the shot1-2 mutant background (Kim et al., 2012, Plant Cell) and verified to be functional based on recovery of shot1 from growth defects. As a control, a transgenic line harboring mitochondria-targeted GFP (Mito-GFP) under the 35S promoter was used. All enriched peaks in SHOT1-GFP samples are located upstream of different tRNA genes: trnS, trnM, trnG and trnF
2020-08-09 | GSE150262 | GEO
Project description:We performed chloroplast ChIP-seq (cpChIP-seq) to identify the possible DNA-binding sites of mTERF5 in Arabidopsis thaliana. To this end, we generated transgenic Arabidopsis plants expressing mTERF5 carrying an HA tag under the control of the CaMV 35S promoter. Then, We used the polyclonal antibody (abcam, ab9110, lot GR304617-8 ) against HA tag which conjugated to ChIP-Grade protein A/G agarose (Thermo scientific, 26161, lot QJ223903) to perform cpChIP assay. The obtained chromatin immunoprecipitated DNA of chloroplasts were used to build DNA libaries for high-throughput sequencing. Finally, we showed that three potenssial DNA regions across the chloroplast genome compared to the control group were enriched by mTERF5.
2019-08-21 | GSE118574 | GEO
Project description:To obtain information on which genes are regulated by an Arabidopsis transcription factor Dof3.2, we treated Arabidopsis transgenic 35S::Dof3.2-GR seedlings with dexamethasone (DEX), then performed DNA microarray analyses. T3 homozygous Dof3.2-GR transgenic line was used.
2020-03-01 | GSE58064 | GEO
Project description:The Arabidopsis thaliana Myb transcription factor, FE, acts as a key regulator of phase transition. In order to identify potential target genes of FE protein, we performed microarray experiments. Using fe-1 and transgenic plants overexpressing GR-tagged FE (35S::FE-GR), we compared transcriptional profiling of WT (L.er) vs fe-1 and Dex-treated 35S::FE-GR vs Mock-treated 35S::FE-GR. Transcriptional profiling of A. thaliana comparing WT (L.er) with the fe-1 mutant
2017-01-01 | GSE42862 | GEO
Project description:Innate immune responses of plant cells confer the first line of defence against pathogens. Signals generated by activated receptors are integrated inside the cell and converge on transcriptional programmes in the nucleus. The Arabidopsis Toll-related intracellular receptor RPS4 operates inside nuclei to trigger resistance to Pseudomonas bacteria expressing AvrRps4 and defence gene reprogramming through the stress response regulator, EDS1. In this immune response, RPS4 cooperates genetically with RRS1 encoding a nuclear TIR-NB-LRR receptor with an additional C-terminal ‘WRKY’ DNA-binding domain. Using transgenic Arabidopsis plants constitutively expressing RPS4 (35S:RPS4), an EDS1-dependent immune response can be turned on rapidly and synchronously in leaf cells after a switch from high (28°C) to moderate (19°C) temperature. In order to determine the relative contributions of RRS1 and EDS1 to temperature-conditioned 35S:RPS4-HS transcriptional reprogramming, we performed gene expression microarray analysis of 35S:RPS4-HS, 35S:RPS4-HS rrs1-11 and 35S:RPS4-HS eds1-2 leaf mRNAs before and after temperature shift. We used transgenic Arabidopsis plants over-expressing RPS4 in EDS1 WT, eds1-2 or rrs1-11 mutant backgrounds. 35S:RPS4, 35S:RPS4 eds1-2 and 35S:RPS4 rrs1-11 plants were grown at 28°C for 3.5 weeks, and subsequently shifted to 19°C. Samples were collected before shift (0h) and 2, 8 and 24h after shift, in triplicates.
2013-12-30 | E-GEOD-50019 | biostudies-arrayexpress
Project description:ngs2014_39_leafnet2-cuc2-identification-Identification of CUC2 targets-10-days old 35S::CUC2-GR Arabidopsis transgenic line grown in liquid culture have been treated for 6 hours with DEXAMETHASONE 10µM or Mock.
2022-05-18 | GSE184530 | GEO
Project description:In order to identify putative downstream target genes of RBE, we sequenced mRNA from dexamethasone (DEX) and mock treated transgenic Arabidopsis line 35S:GR-RBE (RBE coding region fused to a glucocorticoid receptor domain driven by the constitutive 35S promoter) floral tissues. We compared the results from DEX and mock treatments and focused on the 832 genes whose expression was significantly reduced (P < 0.025) by 2-fold or more in DEX as compared to mock-treated plants. In this analysis, we identified MIR164c (EEP1) as a candidate target of RBE, which was further confirmed by other molecular and genetic analyses. Regulation of MIR164c by RBE is important for normal floral organ formation in Arabidopsis.
2012-07-12 | GSE36469 | GEO
Project description:A novel cold-inducible GSK3/Shaggy-like kinase cDNA (TaSK5) was isolated from winter wheat by a macroarray-based differential screening approach. Sequence analysis of TaSK5 revealed high similarity to Arabidopsis subgroup I GSK3/Shaggy-like kinases ASK-alpha, ASK-gamma and ASK-epsilon. Transgenic Arabidopsis plants overexpressing TaSK5 cDNA under the control of CaMV 35S promoter showed enhanced tolerance to salt and drought stresses. In contrast, the tolerance of the transgenic plants to freezing stress was not altered. To identify genes which are differentially regulated in the 35S:TaSK5 over-expressing Arabidopsis plants under non-stress conditions, we compared the genome-wide expression profiles of Col-0 and plants over-expressing TaSK5 using DNA microarrays. Sixty seven genes were found to be expressed at least 2-fold more strongly in 35S:TaSK5 plants than in Col-0, and 17 genes were found to be expressed at least 2-fold more strongly in Col-0 than in 35S:TaSK5 plants. Most of the TaSK5 up-regulated genes were also induced by abiotic stresses, including cold, salt and drought. These results support the involvement of TaSK5 in abiotic stress signal transduction. Keywords: transgenic vs wt Col.-0 comparison
2014-11-02 | GSE59556 | GEO
Project description:To identify the genes regulated by Arabidopsis ERF19. 35S::ERF19 transgenic Arabidopsis was created, them we employ whole genome microarray expression profiling as a discovery platform to identify genes with the potential to regulae by ERF19. The RNA was isopated from the whole flower of control and 35S::ERF19 transgenic Arabidopsis.
2020-11-17 | GSE161564 | GEO