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E-GEOD-59089

ABSTRACT: RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

OTHER RELATED OMICS DATASETS IN: PRJNA254355PRJNA254352PRJNA254354

PROVIDER: E-GEOD-59089 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-GEOD-59089-analysis-methods.tsv	Tabular
	E-GEOD-59089-analytics.tsv	Tabular
	E-GEOD-59089-analytics.tsv.undecorated	Tabular
	E-GEOD-59089-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-59089-analytics.tsv.unrounded	Tabular

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Similar Datasets

Homo sapiens

Project description:RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

| PRJNA254355 | ENA

RNA-seq transcriptional profiling in primary human erythroid progenitor cells upon shRNA-mediated knockdown of PRC2 core subunits

Project description:Polycomb Repressive Complex 2 (PRC2) plays crucial roles in transcriptional regulation and stem cell development. However, the context-specific functions associated with alternative subunits remain largely unexplored. Here we show that the related enzymatic subunits EZH1 and EZH2 undergo an expression switch during hematopoiesis. We examine the in vivo stoichiometry of the PRC2 complexes by quantitative proteomics and reveal the existence of an EZH1-SUZ12 sub-complex lacking EED. We provide evidence that EZH1 together with SUZ12 form a non-canonical PRC2 complex, occupy active chromatin domains in the absence of H3K27me3, and positively regulate gene expression. Loss of EZH2 expression leads to global repositioning of EZH1 chromatin occupancy to EZH2 targets. Moreover, we demonstrate that an erythroid-specific enhancer mediates transcriptional activation of EZH1, and a switch from GATA2 to GATA1 controls the developmental EZH1/2 switch by differential association with EZH1 enhancers during erythropoiesis. Thus, the lineage- and developmental stage-specific regulation of PRC2 expression and subunit composition leads to a switch from canonical silencing to non-canonical PRC2 functions during blood stem cell specification. Transcriptional profiling in primary human fetal liver proerythroblasts upon lentiviral shRNA-mediated knockdown of EZH1, EZH2, EED, or SUZ12 by RNA-seq analysis.

2015-01-01 | E-GEOD-59089 | biostudies-arrayexpress

HDAC1 and HDAC2 in fetal hemoglobin induction

Project description:This SuperSeries is composed of the following subset Series: GSE22366: Primary human erythroid progenitor cells HDAC1 and HDAC2 shRNA knockdown samples GSE22367: Primary human erythroid progenitor cells SAHA treatment samples GSE22368: Primary human erythroid progenitor cells NK57 treatment samples Refer to individual Series

2010-07-29 | E-GEOD-22369 | biostudies-arrayexpress

Homo sapiens

Project description:ChIP-seq analysis of PRC2 core subunits in primary human erythroid progenitor cells

| PRJNA254354 | ENA

Jarid2 regulates hematopoietic stem cell function by acting with Polycomb Repressive Complex 2

Project description:Polycomb Repressive Complex 2 (PRC2) has been shown to play a key role in hematopoietic stem and progenitor cell (HSPC) function. Analyses of mouse mutants harboring deletions of core components have implicated PRC2 in fine-tuning multiple pathways that instruct HSPC behavior, yet how PRC2 is targeted to specific genomic loci within HSPCs remains unknown. Here we use shRNA-mediated knockdown to survey the function of known PRC2 accessory factors in HSPCs by testing the competitive reconstitution capacity of transduced murine fetal liver cells. We find that similar to the phenotype observed upon depletion of core subunit Suz12, depleting Jarid2 enhances the competitive transplantation capacity of both fetal and adult, mouse and human HSPCs. Gene expression profiling revealed common Suz12 and Jarid2 target genes that are enriched for the H3K27me3 mark established by PRC2. These data implicate Jarid2 as an important component of PRC2 that has a central role in coordinating HSPC function. RNA-seq of jarid knockdown, suz knockdown and control from HSPC in 16 week old mice.

2015-04-02 | E-GEOD-60808 | biostudies-arrayexpress

Project description:ChIP-seq and RNA-seq analysis of PRC2 core subunits in primary human erythroid progenitor cells

| PRJNA254352 | ENA

E-MTAB-7284

Project description:RNA-seq of human cancer cell line OVCA420* following TIGAR knockdown with shRNA

2025-01-23 | E-MTAB-7284 | ExpressionAtlas

Homo sapiens

Project description:Primary human erythroid progenitor cells HDAC1 and HDAC2 shRNA knockdown samples

| PRJNA129225 | ENA

E-MTAB-7197

Project description:mRNA-seq of HCT116 RNF40 knockdown cells upon TNF-alpha stimulation

2025-01-23 | E-MTAB-7197 | ExpressionAtlas

E-MTAB-7808

Project description:RNA-seq upon PAX8 knockdown in Renal Cell Carcinoma cell lines

2025-01-23 | E-MTAB-7808 | ExpressionAtlas

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