Transcriptomics,Multiomics

Dataset Information

124

Gene expression profile of CD4-positive T cells from mouse Cbs knockout


ABSTRACT: Hydrogen sulfide (H2S) is an endogenous gasotransmitter and is capable of regulating various endogenous signaling pathways, inculding inflamation and immune response. In mammals, H2S is mainly generated by two pyridoxal-5’-phosphate-dependent enzymes, termed cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE). CBS-deficient mice showed autoimmune disorders. H2S play important roles in T cell development and differentiation, especially Treg cells development and differentiation. We used microarrays to detail the global gene expression of WT and CBS-deficient CD4+ T cells. CD4+ T cells from WT and CBS-deficient mice were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the homogeneous populations of mice at 6 weeks in order to increase the temporal resolution of expression profiles.

REANALYSIS of: E-GEOD-59241

ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-59241 | ExpressionAtlas | 2015-09-18

REPOSITORIES: ExpressionAtlas

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Publications

Hydrogen Sulfide Promotes Tet1- and Tet2-Mediated Foxp3 Demethylation to Drive Regulatory T Cell Differentiation and Maintain Immune Homeostasis.

Yang Ruili R   Qu Cunye C   Zhou Yu Y   Konkel Joanne E JE   Shi Shihong S   Liu Yi Y   Chen Chider C   Liu Shiyu S   Liu Dawei D   Chen Yibu Y   Zandi Ebrahim E   Chen Wanjun W   Zhou Yanheng Y   Shi Songtao S  

Immunity 20150811 2


Regulatory T (Treg) cells are essential for maintenance of immune homeostasis. Here we found that hydrogen sulfide (H2S) was required for Foxp3(+) Treg cell differentiation and function and that H2S deficiency led to systemic autoimmune disease. H2S maintained expression of methylcytosine dioxygenases Tet1 and Tet2 by sulfhydrating nuclear transcription factor Y subunit beta (NFYB) to facilitate its binding to Tet1 and Tet2 promoters. Transforming growth factor-β (TGF-β)-activated Smad3 and inte  ...[more]

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