Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-61636

ABSTRACT: Transcription profiling by high throughput sequencing of murine bone marrow endothelial cells and bone marrow stroma, in vitro and in vivo, with and without hematopoietic stem cells co-culture

PROVIDER: E-GEOD-61636 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-61636-analysis-methods.tsv	Tabular
	E-GEOD-61636-analytics.tsv	Tabular
	E-GEOD-61636-analytics.tsv.undecorated	Tabular
	E-GEOD-61636-analytics.tsv.undecorated.unrounded	Tabular
	E-GEOD-61636-analytics.tsv.unrounded	Tabular

Items per page:

1 - 5 of 52

Similar Datasets

E-MTAB-6849

Project description:RNAseq of human bone marrow mesenchymal stem cells in co-culture with endothelial cells

2025-01-23 | E-MTAB-6849 | ExpressionAtlas

E-GEOD-42257

Project description:Murine bone marrow gene expression profiling

2015-08-06 | E-GEOD-42257 | ExpressionAtlas

EGR1 regulation in human bone marrow stromal cells

Project description:Human bone marrow stromal cells (BMSCs) are key elements of the hematopoietic environment and they play a central role in bone and bone marrow physiology. However, how key BMSC functions are regulated is largely unknown. We analyzed the role of the immediate early response transcription factor EGR1 as key BMSC regulator and found that EGR1 was highly expressed in prospectively-isolated primary BMSCs, downregulated upon culture, and lower in non-CFU-F-containing CD45neg BM cells. Furthermore, EGR1 expression was lower in proliferative regenerating adult and fetal primary cells compared to adult steady-state BMSCs. Accordingly, EGR1 overexpression markedly decreased BMSC proliferation but considerably improved hematopoietic stroma support function as indicated by an increased production of transplantable CD34+CD90+ hematopoietic stem cells in expansion co-cultures. The improvement of BMSC stroma support function was mediated by increased expression of hematopoietic supporting genes, such as VCAM1 and CCL28. On the other hand, EGR1 knockdown increased ROS-mediated BMSC proliferation, and clearly reduced BMSC hematopoietic stroma support potential. These findings thus show that EGR1 is a key BMSC transcription factor with a dual role in regulating proliferation and hematopoietic stroma support function that is controlling a genetic program to coordinate the specific functions of BMSC in their different biological contexts.

2019-06-27 | PXD011767 | Pride

E-GEOD-1571

Project description:Gene expression of side population and main population cells, isolated from adult murine skeletal muscle and bone marrow

2015-09-03 | E-GEOD-1571 | ExpressionAtlas

Bone marrow stroma confers breast cancer cell resistant to cisplatin treatment

Project description:The transcriptome changes of breast cancer cell SUM1315, with or without co-culture with bone marrow stroma cells, upon cisplatin (CDDP) treatment.

2017-12-27 | GSE97997 | GEO

Isolation and functional characterization of the arteriole hematopoietic stem cell niche

Project description:Hematopoietic stem cells (HSCs) inhabit distinct microenvironments within the adult bone marrow (BM) that govern the delicate balance between HSC quiescence, self-renewal, and differentiation. It has been suggested that quiescent HSCs localize adjacent to BM arteriole endothelial cells in a significant and non-random distribution. This data suggests that the arteriole BM vascular niche may be the primary HSC niche. Because the BM arteriole niche is composed of tightly-associated pericytes, including smooth muscle actin+, LepR+, Nestin+, NG2+, and nonmyelinating Schwann cells, we sought to begin to uncouple the arteriole BM EC niche by examining its capacity to support the maintenance and expansion of HSCs ex vivo and in vivo. We developed a method to isolate and culture BM arteriole endothelial cells in serum-/growth factor-free conditions, allowing for a non-biased approach to examining their instructive function. Utilizing our protocol, we demonstrate that BM endothelial cells, but not BM stromal cells, have the capacity to expand long-term repopulating, multi-lineage HSCs in lieu of complex serum and cytokine supplementation. In addition, transplantation of arteriole endothelial cells promoted rapid hematopoietic recovery and protected HSCs following an LD50 dose of myeloablative irradiation. These data demonstrate that arteriole-derived BM endothelial cells are endowed with the necessary signals to support the self-renewal and regenerative capacity of LT-HSCs and that transplantation of arteriole BM endothelial cells could be used as a therapeutic means to decrease pancytopenias associated with myeloablative treatments to treat a wide array of disease states. Transcriptome sequencing of bone marrow endothelial cells and bone marrow stroma, in vitro and in vivo, with and without HSC co-culture.

2015-11-04 | E-GEOD-61636 | biostudies-arrayexpress

E-GEOD-55014

Project description:Identification of candidate genes regulated by E4bp4 in murine bone marrow

2015-08-04 | E-GEOD-55014 | ExpressionAtlas

E-GEOD-63569

Project description:RNA-seq of bone marrow CD34+ cells from myelodysplastic syndrome patients with and without SF3B1 mutation and from healthy controls

2025-01-24 | E-GEOD-63569 | ExpressionAtlas

E-MTAB-1963

Project description:Transcription profiling by high throughput sequencing between multipotent hematopoietic stem progenitor cells LS+K) and myeloid committed cells (LSK) of the mouse bone marrow

2019-05-14 | E-MTAB-1963 | ExpressionAtlas

E-GEOD-22434

Project description:Expression data from Evi1-transduced primary bone marrow cells

2015-09-15 | E-GEOD-22434 | ExpressionAtlas