Project description:Lysine malonylation is a kind of post-translational modifications (PTMs) discovered in recent years, which plays an important regulatory role in plants. Maize (Zea mays L.) is a major global cereal crop. We therefore performed a global malonylome analysis of maize. In total, 1722 uniquely malonylated lysine residues were obtained in 810 proteins. The modified proteins were involved in various biological processes such as photosynthesis, ribosome and oxidative phosphorylation. Notably, a large proportion of the modified proteins (45%) were located in chloroplast, suggesting an indispensable regulatory role of malonylation in photosynthesis and carbon fixation.
Project description:We report the application of whole transcriptome sequencing technology for high-throughput profiling of coding and non-coding RNAs associated with Spodoptera frugiperda feeding in Zea mays. 4,366 mRNAs and 233 lncRNAs were differentially expressed during Spodoptera frugiperda feeding in Zea mays. Our data contribute to the understanding of the function of coding and non-coding RNAs in the regulation of plant-insect interactions.
Project description:The differentiation of specialized feeding sites in Zea mays root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Zea mays root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation.
Project description:MicroRNAs (miRNAs) are endogenous small noncoding RNAs (18–25 nt) that are involved in many physiological processes including development, cancer, immunity, apoptosis and host-microbe interactions through posttranscriptional regulation of gene expression. In this study, we measured the profile of small RNAs in Zea mays after one day and three days of Spodoptera frugiperda feeding. We identified 500 miRNAs, including 449 known and 51 novel miRNAs. In addition, we identified the miRNAs differentially expressed in Z. mays after one day and three days of S. frugiperda feeding, and the possible target genes were identified. This study identified critical miRNAs involved in the Z. mays during S. frugiperda feeding, thus providing a useful resource for exploring the regulatory role of miRNAs during plant-insect interactions.
Project description:Transcriptome profiling of Pseudomonas putida KT2440 comparing cells exposed for 1 hour to DIMBOA from maize (Zea mays) to unexposed cells
Project description:Microarray hybridization was used to assess acclimation responses to four UV regimes by near isogenic Zea mays lines varying in flavonoid content. Keywords: other
Project description:In maize (Zea mays), irradiation can produce many variants with varying centromeric DNA sequences. We found de novo centromeres in F3-152 and F3-600, which has no canonical centromeric repeat sequences. This centromere is derived from a 1520-kb and 188-kb region on the arm of chromosome 10 and 3. Our results suggest that de novo centromere formation is more common than previously thought and can persist on chromosomal fragments without a canonical centromere providing implications for karyotype evolution.