Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-GEOD-6400

ABSTRACT: Transcription profiling of human A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050 a new class of tumor-selective inhibitors of gene expression.

OTHER RELATED OMICS DATASETS IN: PRJNA99711

PROVIDER: E-GEOD-6400 | ExpressionAtlas | 2025-01-24

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-GEOD-6400-analysis-methods.tsv	Tabular
	E-GEOD-6400-atlasExperimentSummary.Rdata	Rdata
	E-GEOD-6400-configuration.xml	Xml
	E-GEOD-6400-percentile-ranks.tsv	Tabular
	E-GEOD-6400.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 89

Similar Datasets

Transcription profiling of human A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050 a new class of tumor-selective inhibitors of gene expression.

2007-10-26 | E-GEOD-6400 | biostudies-arrayexpress

Cultured A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050

Project description:The goal of this project was to characterize changes in gene expression in response to the anti-cancer agent sapphyrin PCI-2050. Cultured A549 human lung cancer cells were treated with sapphyrin PCI-2050 or actinomycin D, a known transcripitonal inhibitor. The gene expression profiles of drug-treated and control A549 cultures were determined using Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA). Further details are provided in our published manuscript: <http://www.molecular-cancer.com/content/6/1/9>. Keywords: responses to treatments with anti-cancer agents

2006-12-01 | GSE6400 | GEO

Homo sapiens

Project description:Cultured A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050

| PRJNA99711 | ENA

E-GEOD-2189

Project description:Transcription profiling of human lung cancer cell A549 lines treated with the chemotherapeutic drug motexafin gaolinium at 4, 12 and 24h timepoints

2025-01-24 | E-GEOD-2189 | ExpressionAtlas

E-GEOD-26525

Project description:Expression data analyzed with LPIA in A549 lung carcinoma cells treated with geldanamycin.

2025-01-24 | E-GEOD-26525 | ExpressionAtlas

E-MTAB-5627

Project description:Microarray analysis of human A549 lung cancer cells treated with brefeldin A, golgicide A, monensin or vehicle control for 8 h or 20 h

2025-01-23 | E-MTAB-5627 | ExpressionAtlas

Synthesis and Anticancer Properties of Water-Soluble Zinc Ionophores 1

2007-02-07 | GSE6960 | GEO

E-MTAB-8572

Project description:RNA-seq of human lung cancer cell line A549-NC and A549-SLC2A5 knockout xenograft in balb/c nude mice.

2025-01-16 | E-MTAB-8572 | ExpressionAtlas

Evaluation of genetic inactivation of lysine deacetylases KDAC6 (HDAC6) and KDAC8 (HDAC8) versus treatment with KDAC-selective inhibitors

Project description:To investigate the specificity and biological impacts of KDAC-selective inhibitors, we treated wild-type HT1080 cells and cell lines containing point mutations that result in endogenous expression of a catalytically-inactive variant with KDAC6 and KDAC8 selective inhibitors Tubastatin A and PCI-34051. Expression profiling analysis was then performed on the treated cells vs. untreated wild HT1080 cells.

2024-02-15 | GSE251743 | GEO

Transcription profiling of A549 lung cancer cultures treated with the lead compound PCI-5002 supplemeted with zinc reveals synthesis and anticancer properties of water-soluble zinc ionophores

Project description:We have shown that water solubilized versions of a zinc ionophore increase intracellular concentrations of free zinc and have antiproliferative activity in exponential phase A549 lung cancer cultures. The gene expression profiles of A549 lung cancer cultures treated with the lead compound PCI-5002 reveal the activation of stress response pathways. Medium supplementation with zinc (25 Î¼M) led to activation of additional oxidative stress response as well as apoptotic pathways. We propose that the pharmacologic delivery of zinc to tumors using water solubilized ionophores is a potential approach to cancer therapy. Experiment Overall Design: A549 human lung cancer cells (1 x 105 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, sapphyrin PCI-5002 (10 Î¼M final concentration), ZnOAc2 (25 Î¼M final concentration), the combination, or control (5% mannitol) solution was added to the cultures. Each experiment was performed in triplicate. After incubation, all cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated and subjected to analysis on Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA), as described. ArrayAssist software (Stratagene/Affymetrix) and the RMA (Robust Microarray Analysis) algorithm were used to generate scaled gene expression values.

2008-06-15 | E-GEOD-6960 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data