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E-GEOD-66290

ABSTRACT: WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

OTHER RELATED OMICS DATASETS IN: PRJNA276454PRJNA276444PRJNA276445

PROVIDER: E-GEOD-66290 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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Arabidopsis thaliana

Project description:WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

| PRJNA276444 | ENA

WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. We identified by ChIP-seq >1680 high-confidence WRKY33 binding sites associated with 1576 genes within the Arabidopsis genome, with all of them being dependent on rapid activation of WRKY33 expression by Botrytis cinerea strain 2100. Genome-wide transcriptional analysis defined 318 genes as direct functional targets at 14 h post inoculation. Comparison between resistant wild-type Columbia-0 and susceptible wrky33 mutant plants revealed that expression of 75% of all WRKY33 regulated targets were down-regulated upon infection, indicating that WRKY33 predominately acts as a repressor. However, WRKY33 appears to possess dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. Our genome-wide analysis confirmed known WRKY33 targets involved in ethylene and jasmonic acid hormone signaling and phytoalexin biosynthesis, but also uncovered a previously unknown role of abscisic acid (ABA) biosynthesis in the complex regulatory circuitry affecting resistance towards Botrytis. Analysis of transgenic plants expressing WRKY33-HA under its native promoter post inoculation with spores of Botrytis cinerea 2100

2015-06-15 | E-GEOD-66289 | biostudies-arrayexpress

Dataset for proteome analysis of epidermis of Arabidopsis leaves inoculated with Fusarium graminearum

Project description:We analyzed protein expression of Arabidopsis leaf epidermis after inoculation of Fusarium graminearum.

2023-01-21 | PXD039595 | JPOST Repository

Project description:Analysis of WRKY33 binding sites and WRKY33-dependent gene expression in Arabidopsis thaliana upon Botrytis cinerea 2100 inoculation

| PRJNA276454 | ENA

WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

2015-06-15 | GSE66289 | GEO

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100. Comparison of altered gene expression in Arabidopsis WT and wrky33 mutant plants 14 hours post inoculation with Botrytis cinerea 2100.

2015-06-15 | E-GEOD-66290 | biostudies-arrayexpress

Nematode infection migratory stage

Project description:This experiment analyzes the changes in expression of twelve days old Arabidopsis roots at ten hours post inoculation upon cyst nematode H. schachtii infection.

2017-03-23 | E-MTAB-5610 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

| PRJNA276445 | ENA

Global variation of gene expression induced by constitutive activation of the NIK receptor in Arabidopsis

Project description:Arabidopsis was transformed with the NIK receptor or the mutant T474D NIK receptor and infected with CaLCuV. The global variation of gene expression was analysed by RNA-seq at 10 days post inoculation and 20 days post inoculation and differential gene expression was determinated in comparison with mock inoculated Arabidopsis lines.

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WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

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