Transcriptomics

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Transcription profiling by high throughput sequencing of three populations of primary dentate gyrus cells


ABSTRACT: Lysophosphatidic acid receptor 1 (LPA1) is a phospholipid which has been linked to adult hippocampal neurogenesis and learning deficits. Here we investigated whether LPA acts directly on the hippocampal stem cells and whether LPA1 could be a functional marker for their prospective isolation. Our results reveal that exogenous LPA increases precursor potential in vitro and net neurogenesis in vivo, an effect that is mediated by Akt activation. Using a mouse reporter line, immunohistochemistry and flow cytometry followed by in vitro cell culture, we show that, in contrast to the subventricular zone, neural precursor cells in the adult mouse dentate gyrus express LPA1-GFP. Fluorescence-activated cell sorting for LPA1-GFP in combination with Prominin-1 and epidermal growth factor receptor expression allowed the efficient isolation of a very pure population of hippocampal stem cells. Transcriptional analysis revealed a profile suggesting immune response and cytokine signaling as molecular regulators of adult hippocampal neural stem cells. RNA from cells sorted (FACS) from the dentate gyrus of adult LPA1-GFP (lysophosphatidic acid receptor) reporter mice. Sorting was based on the markers EGFR (Egfr), LPA1 (Lpar1) and Prominin1 (Prom1). From each of 4 replicates, 3 populations (stem cells, progenitor cells, niche cells) were sequenced.

REANALYSIS of: E-GEOD-68270

ORGANISM(S): Mus musculus  

PROVIDER: E-GEOD-68270 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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Publications

Lysophosphatidic Acid Receptor Is a Functional Marker of Adult Hippocampal Precursor Cells.

Walker Tara L TL   Overall Rupert W RW   Vogler Steffen S   Sykes Alex M AM   Ruhwald Susann S   Lasse Daniela D   Ichwan Muhammad M   Fabel Klaus K   Kempermann Gerd G  

Stem cell reports 20160331 4


Here, we show that the lysophosphatidic acid receptor 1 (LPA1) is expressed by a defined population of type 1 stem cells and type 2a precursor cells in the adult mouse dentate gyrus. LPA1, in contrast to Nestin, also marks the quiescent stem cell population. Combining LPA1-GFP with EGFR and prominin-1 expression, we have enabled the prospective separation of both proliferative and non-proliferative precursor cell populations. Transcriptional profiling of the isolated proliferative precursor cell  ...[more]

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