Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-MEXP-1179

ABSTRACT: Transcription profiling of Drosophila expressing four mutant forms of MYOC (R342K, Q368X, D380N and K423E) to model Myocilin-Associated Glaucoma

PROVIDER: E-MEXP-1179 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	E-MEXP-1179-analysis-methods.tsv	Tabular
	E-MEXP-1179-atlasExperimentSummary.Rdata	Rdata
	E-MEXP-1179-configuration.xml	Xml
	E-MEXP-1179-percentile-ranks.tsv	Tabular
	E-MEXP-1179.condensed-sdrf.tsv	Tabular

Items per page:

1 - 5 of 133

Similar Datasets

Transcription profiling of Drosophila expressing four mutant forms of MYOC (R342K, Q368X, D380N and K423E) to model Myocilin-Associated Glaucoma

Project description:We have established Drosophila melanogaster as a model system for ocular hypertension by expressing wild-type human myocilin (MYOC) in the Drosophila eye. Here, we have created transgenic flies that express four clinically relevant mutant forms of MYOC (R342K, Q368X, D380N and K423E) in their eyes using the gmr-Gal4/UAS binary system. We compare and identify human glaucoma candidate genes based on the transcription profiles of flies that express wt-MYOC or mutant-MYOCs.

2008-07-13 | E-MEXP-1179 | biostudies-arrayexpress

Project description:mRNA sequencing of mutant Huntingtin expressing Drosophila

| PRJNA982830 | ENA

Persistent Epigenetic Reprogramming of Sweet Taste by Diet

Project description:We report the application of ribosomal profiling based RNA sequencing technology for high-throughput profiling of the Gr5a cells of male Drosophila melanogaster adults. By expressing the UAS-Rpl3-3XFLAG transgene using the Gr5a-GAL4 driver on Pclc429 mutant flies.

2020-11-11 | GSE146241 | GEO

Project description:Small RNA sequencing of mutant Huntingtin expressing Drosophila

| PRJNA658796 | ENA

Transcription profiling by array of Drosophila embryonic chordotonal neurons expressing the cato GFP reporter gene construct

Project description:Further to a previous transcription profiling experiment using a GFP reporter gene construct expressing the Drosophila proneural gene atonal (Cachero et al. 2011), a later expressing transcription factor and downstream target of ato, cato (cousin of ato) was explored as the GFP reporter in this experiment. cato-GFP expressing cells were isolated from 10h45min and 11h45min old embryos. GFP+ and GFP- cells were compared on Affymetrix Drosophila version 2.0 chips.

2019-02-19 | E-MTAB-7529 | biostudies-arrayexpress

Expression data from larvae body wall expressing LaminC transgenes

Project description:Expression of mutant lamins in human muscle causes muscular dystrophy. We have generated a drosophila model that expresses mutant lamins, modeled after those that cause disease in humans. We used affymetrix microarrays to determine the global changes in gene expression caused by mutant lamins. Dissected 3rd instar larval body wall from transgenic Drosophila expressing either a full length wildtype Lamin C, an N-terminal Lamin C truncation of the first 42 amino acids or a single amino acid substitution in Lamin C at position 489 from a G residue to a V residue under control of the Gal4-UAS. RNA was extracted from 3rd instar larval body wall expressing these mutant Lamin C transgenes and microarray analysis using Affymetrix microarrays.

2015-04-16 | E-GEOD-49959 | biostudies-arrayexpress

Personalized Cancer Therapy for Patients With Metastatic Medullary Thyroid or Metastatic Colon Cancer

Project description:The Personalized Discovery Process is the only program offering patients treatment recommendations based on an empirically constructed Drosophila "fly" model of their disease. Special committee selects one of the one of the few 2-3 FDA approved drug combinations or single agents that improved survival in the fly cancer model.

| 2183852 | ecrin-mdr-crc

Global quantitative proteomic analysis of aging Drosophila eyes

Project description:Wild-type Drosophila melanogaster expressing nuclear GFP-KASH fusion protein in photoreceptors for cell type-specific gene expression profiling (Rh1-Gal4>UAS-GFPKASH ; Genotype = w1118;; P{w+mC=[UAS-GFP-Msp300KASH}attP2, P{ry+t7.2=rh1-GAL4}3, ry506) were raised in 12:12h light:dark cycle at 25°C. Flies were aged for 10 or 40 days post-eclosion, and eyes were harvested from male flies for global quantitative proteomic analysis. Significantly changed proteins were identified that may contribute to age-associated retinal degeneration and loss of visual function in the aging Drosophila eye.

2021-07-23 | PXD027090 | Pride

Human HNF1A KI mice liver proteome analysis

Project description:Nonalcoholic fatty liver disease (NAFLD) is a pathological condition caused by excessive fat accumulation in liver. HNF1A is the pathogenic cause of hepatocellular adenoma and HCC. It is an important transcription factor in liver. We constructed a knock-in mice expressing a human dominant negative HNF1α P291fsinsC mutation to explore how the pathogenic mutant affected lipid metabolism. Three liver samples of control group and four liver samples of KI group were used to do proteome analysis.

2023-03-02 | PXD040561 |

Profiling Drosophila third instar larval eye discs with disruptions in Hippo and Rbf pathways by 10X single-cell RNA-Sequencing

Project description:We performed single-cell RNA-sequencing experiments to understand how the Rbf and Hippo pathways collaborate to maintain cell identity. Drosophila melanogaster photoreceptor neurons with dual mutation in Rbf[120a] and wts[x1] specify normally before abruptly dedifferentiating. We performed scRNA-Seq on w1118, Rbf[120a] single mutant, wts[x1] single mutant, and Rbf[120a] wts[x1] double mutant tissues to better understand the dedifferentiation phenotype. We identified transcriptional changes that were unique to double mutant tissue. We then validated these experiments using a novel dedifferentiation model expressing constitutively active yki[S111A,S168A,S250A] in Rbf[120a] mutant tissue using the GMR-GAL4 driver. We performed single-cell experiments on eye tissues expressing yki[S111A,S168A,S250A] in Rbf wildtype and mutant backgrounds, as well as tissues co-expressing Hth and yki. We then analyzed these experiments to determine the dedifferentiation transcriptional program.

2023-08-04 | GSE217380 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data