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E-MEXP-1179

ABSTRACT: Transcription profiling of Drosophila expressing four mutant forms of MYOC (R342K, Q368X, D380N and K423E) to model Myocilin-Associated Glaucoma

PROVIDER: E-MEXP-1179 | ExpressionAtlas | 2015-10-22

REPOSITORIES: ExpressionAtlas

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Transcription profiling of Drosophila expressing four mutant forms of MYOC (R342K, Q368X, D380N and K423E) to model Myocilin-Associated Glaucoma

Project description:We have established Drosophila melanogaster as a model system for ocular hypertension by expressing wild-type human myocilin (MYOC) in the Drosophila eye. Here, we have created transgenic flies that express four clinically relevant mutant forms of MYOC (R342K, Q368X, D380N and K423E) in their eyes using the gmr-Gal4/UAS binary system. We compare and identify human glaucoma candidate genes based on the transcription profiles of flies that express wt-MYOC or mutant-MYOCs.

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