Project description:we focused on characterizing proteomicchanges involving JAZ7 when Arabidopsis thaliana plants were withhold water. To understand the phenotypes and elucidate the regulatory function of JAZ7, leaves from wild type(WT), jaz7 knock out(KO), jaz7 overexpression(OE)were harvested for proteomics analysis after drought treatment for 18days. Using TMT-based isobaric labeling quantitative proteomics approach,
Project description:The goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.
Project description:Transcriptional profiling of Arabidopsis thaliana, comparing control wild-type (ecotype Wassilewskija, Ws) leaves with leaves from transgenic plants overexpressing the transcription factor RAP2.6L under control of the cauliflower mosaic virus 35S promoter (RAP2.6L-OX; this line was originally described in Krishnaswamy et al (2010)).
Project description:Gene expression profiling of knock out mutant NOG1-1 and RNAi line NOG1-2 was studied in comparison to wild type in leaves of seven days old plants.
Project description:au07-13_sqe1 - sqe1 - Arabidopsis transcriptome microarray from wild type and mutant plants. - Arabidopsis microarray from Ler ecotype and the sqe-1 mutant. Keywords: gene knock out
Project description:Analysis of transcriptional profiling of Arabidopsis Col-0 leaves from wild-type and plants with incresed levels of AtOXR2 (oeOXR2 plants)
Project description:RNA-sequencing performed on petals and inflorescence of Arabidopsis plants. The study provides insight into the role of the TCP5 transcription factor and its molecular mechanism underlying petal growth, using knock-out, overexpression and induction lines on which RNA-sequencing was performed.
Project description:ABHD11 has a acylhydrolase activity. Knock-out of abhd11 increases phospholipids in plant leaves. Wholegenome array was done to check the differential expressed genes in lipid metabolic pathway
Project description:Brassica oleraceae plants were treated with jasmonic acid either at the roots or two leaves. An acidic (HCl) water sollution with the same pH as the jasmonic acid sollution was also applied to two leaves of the root jasmonic acid treated plants, and to the roots of leaf jasmonic acid treated plants. Control plants received a mock treatment on roots and leaves with acidic water of the same pH as the jasmonic acid sollution. The whole root system and two systemic leaves were harvested separately at 6, 18 and 30 h after treatment. For each time point, tissue and treatment, three biological replicates (except two replicates for 'Root-Cont-18h' sample) were made consisting of pooled tissue samples from 10 plants. Gene expression was analyzed in both tissues using the 29,000 element Arabidopsis Oligonucleotide Microarrays (Qiagen-Operon Arabidopsis Genome Array Ready Oligo Set version 3.0).