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E-MTAB-1929

ABSTRACT: Transcriptional regulation of endocrine cell differentiation by Insm1

PROVIDER: E-MTAB-1929 | ExpressionAtlas | 2019-05-14

REPOSITORIES: ExpressionAtlas

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			Action	DRS
	E-MTAB-1929-analysis-methods.tsv	Tabular
	E-MTAB-1929-analytics.tsv	Tabular
	E-MTAB-1929-analytics.tsv.undecorated	Tabular
	E-MTAB-1929-analytics.tsv.undecorated.unrounded	Tabular
	E-MTAB-1929-analytics.tsv.unrounded	Tabular

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Transcriptional regulation of endocrine cell differentiation by Insm1

Project description:Transcriptional regulation of endocrine cell differentiation by Insm1

| PRJEB4758 | ENA

Genome-wide analysis of E17.5 pituitary gland gene expression of control and Insm1 mutant mice

Project description:The Insm1 gene encodes a zinc finger factor expressed in many endocrine organs. We show here that Insm1 is required for differentiation of all endocrine cell types in the pituitary. Thus, in Insm1 mutant mice, hormones characteristic of the different pituitary cell types (thyroid, follicle and melanocyte stimulating hormone, adrenocorticotrope hormone, growth hormone and prolactin) are absent or produced at markedly reduced levels. The differentiation deficit is accompanied by an up-regulated expression of components of the Notch signaling pathway. Further, skeletal muscle-specific genes are ectopically expressed, indicating that Insm1 blocks a muscle-specific expression program. Since Insm1 is also essential for differentiation of endocrine cells in the pancreas, intestine and adrenal gland, it is emerging as a transcription factor that acts in a pan-endocrine manner. The Insm1 factor contains a SNAG domain at its N-terminus, and we show here that the SNAG domain recruits histone modifying factors (Kdm1a, Hdac1/2 and Rcor1-3) and other proteins implicated in transcriptional regulation (Hmg20a/b and Gse1). Deletion of the SNAG domain in mice disrupted differentiation of pituitary endocrine cells, and resulted in an upregulated expression of components of the Notch signaling pathway and ectopic expression of skeletal muscle-specific genes. Our work demonstrates that Insm1 acts in the transcriptional network that controls differentiation of endocrine cells in the anterior pituitary gland, and requires the SNAG domain to exert this function in vivo. Analysis of genes regulated by Insm1 in embryonic day 17.5 pituitary gland. Total RNA from pituitary glands of E17.5 control embryos was compared to E17.5 Insm1 mutant embryos.

2013-10-20 | E-GEOD-46139 | biostudies-arrayexpress

E-MTAB-3352

Project description:Transcriptional profiling of Ngn3-dependent pancreatic endocrine differentiation at E15.5

2019-05-14 | E-MTAB-3352 | ExpressionAtlas

Genome-wide analysis of E17.5 pituitary gland gene expression of control and Insm1 mutant mice

2013-10-20 | GSE46139 | GEO

Transcriptional regulation of endocrine cell differentiation by Insm1

Project description:This experiment used RNA-Seq technology to explore gene expression in mouse Insm1^GFP/Pdx1^CFP HIGH [het/het] FACS sorted pancreatic cells (pre-beta cells) and Insm1^GFP/Pdx1^CFP LOW [het/het] cells (other endocrine progenitors) at E15.5 and E18.5. Comparison of Insm1 +/Pdx HIGH and Insm1 +/Pdx LOW cells revealed a set of differentially expressed genes that are required for beta cell specification.

2014-07-01 | E-MTAB-1929 | biostudies-arrayexpress

Thymic mimetic cells function beyond self-tolerance [Insm1]

Project description:To examine the Endocrine thymic epithelial cell transcriptome within the mTEC-III compartment we sorted this population from young Insm1 cKO mice and performed bulk RNAseq

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E-MTAB-7345

Project description:RNASeq: Aryl hydrocarbon receptor governs a transcriptional programme that determines regulatory B cell differentiation and function

2020-01-10 | E-MTAB-7345 | ExpressionAtlas

RNA-Seq analysis of E15.5 mouse Insm1, Neurod1 and Pax6 knockouts vs Insm1GFP.Cre/+ controls

Project description:This experiment used RNA-Seq technology to explore gene expression in mouse Insm1GFP.Cre/+ controls and Insm1 (Insm1GFP.Cre/GFP.Cre), Neurod1 (Insm1GFP.Cre/+; Neurod1LacZ/LacZ), or Pax6 (Insm1GFP.Cre/+; Pax6fl/fl) knockout FACS sorted pancreatic endocrine cells at E15.5. Comparison of Insm1GFP.Cre+/- and knockout animals revealed sets of differentially expressed genes that are required for endocrine cell specification and development.

2021-08-23 | E-MTAB-10262 | biostudies-arrayexpress

E-MTAB-9250

Project description:Glucocorticoids induced transcriptional regulation in acute lymphoblastic leukemia

2025-01-16 | E-MTAB-9250 | ExpressionAtlas

Insm1 cooperates with Neurod1 and Foxa2 to maintain mature pancreatic β-cell function (Insm1, Neurod1 and Foxa2 ChipSeq data from pancreatic beta cell)

Project description:Genome-wide Insm1 binding sites analysis revealed an overrepresentation of sequences predicted to bind bHLH and/or forkhead factors, and further analyses demonstrated that most Insm1 sites in the genome are co-occupied by Neurod1 and Foxa2. Binding regions co-occupied by all three factors but not single Insm1 sites explained a significant fraction of gene expression changes in Insm1 mutant β-cells. Together, our data provide evidence that an Insm1, Neurod1 and Foxa2 network maintains a mature gene expression program in β-cells. 6 samples include, replicates of Insm1 and Foxa2, IgG pull down as a control

2015-03-31 | E-GEOD-54046 | biostudies-arrayexpress

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