Project description:About 15-20% of all breast cancers are triple negative breast cancers, which are often highly aggressive. We performed global quantitative phosphotyrosine profiling of a large panel of triple negative breast cancer cell lines using high resolution Fourier transform mass spectrometry. Our study identified 1,903 tyrosine-phosphorylated peptides derived from 969 proteins. Heterogeneous activation of tyrosine kinases was observed in triple negative breast cancer derived cell lines.
Project description:In this work of Kisaki et al, we analyze the variation of proteome responses upon treatment of breast cancer cell lines MCF7 and MDA-MB231 with Bothrops jararaca snake venom
Project description:We aim to test whether our panel of breast cancer cell lines belong to the claudinlow subtype of breast cancer using the classifier published by Prat et al. Breast Cancer Research 12, 2010. For the analysis, we used gene expression data of our cell lines obtained with Illumina bead arrays grown in 2D as attahed and in 3D as mammosphere cultures.
Project description:Baseline gene expression for cell lines growing in culture were measured by Affymetrix U133Plus2 arrays. Copy Number Variation for selected cell lines was also measured. Baseline gene expression for cell lines growing in culture were measured by Affymetrix U133Plus2 arrays. Three different sources of arrays were combined. Arrays were from Pfizer, Wyeth Breast Panel, and Wyeth NCI50 subset of NCI60 cell lines. CNV was determined by Affymetrix SNP6.0 arrays.
Project description:Expression profiling of a panel of urothelial cancer cells. The goal of the study is to exam the genome wide expression profile in each of the 30 urothelial cancer cells tested in our laboratory Each of the 30 cell lines was DNA fingerprinted to confirm its real identity. Total RNA was obtained from each cell line and subjected to illumina expression profiling microarray
Project description:We compared the gene expression profile of 26 breast cancer cell lines to identify variations associated with different phenotypes and molecular subtype. The panel includes 13 estrogen receptor negative (ER-) and 13 ER+ cell lines. Four of the ER- cell lines and 5 of the ER+ cell lines are HER2 positive.
Project description:RNA-sequencing analysis of CDK12 overexpressiong ZR-75-30 cell lines. Cyclin dependent kinase 12 (CDK12) is amplified in approximately 70-90% of HER2 amplified breast cancer. Results provide insight into the targets of CDK12 in HER2 positive breast cancer.