Project description:Whole genome transcriptional profiling of human monocyte derived macrophages differentiated in the presence of human or foetal calf serm
Project description:Transcriptional profiling of Aza+ITF-2357 treated bone marrow derived macrophages vs. Mock treated bone marrow derived macrophages. Aim was to elucidate the effects of epigentic drug treatment on in vitro cultured macrophages and to compare these data to in vivo treated macrophage populations. Transcriptional profiling of NSCLC cell lines treated with epigenetic agents Transcriptional profiling of immune populations in the lung Kras G12D tumor micronevironment treated with combination epigenetic therapy Transcriptional profiling of lung Kras G12D tumors treated with combination epigenetic therapy
Project description:Transcriptional profiling of primary human blood-derived macrophages (BDMs) comparing control untreated BDMs with BDMs exposed with Streptococcus pneumoniae strain D39 (MOI 0.1 and 0.5) for 16 hours)
Project description:This SuperSeries is composed of the following subset Series: GSE19482: Transcriptional responses of human monocyte-derived macrophages (HMDM) to lipopolysaccharide (LPS) GSE19490: Transcriptional responses of mouse BMM and TEPM to lipopolysaccharide (LPS) GSE19765: Transcriptional responses of human monocyte-derived macrophages (HMDM) to lipopolysaccharide (LPS) - Illumina arrays GSE19766: Transcriptional responses of mouse bone marrow-derived macrophages (BMM) to lipopolysaccharide (LPS) - Illumina arrays Refer to individual Series
Project description:Recent efforts have uncovered immense transcriptional and ontogenetic diversity among tissue-resident macrophages, each with their own transcriptional profile endowing the cell with its tissue-specific functions. However, it is currently unknown whether the origins of different macrophage populations may affect their roles in malignancy. Given potential artifacts associated with irradiation-based lineage tracing, it remains unclear if bone marrow-derived macrophages (BMDM) are even present in tumors of the brain, a tissue where there is no homeostatic involvement of peripherally-derived myeloid cells. Here, we employed multiple models of murine brain malignancy and genetic lineage tracing models to demonstrate that BMDM are indeed abundant in primary and metastatic brain tumors. Transcriptional profiling of tumor-associated BMDM and resident microglia showed that these cells acquire substantially different gene expression profiles. Our data suggest that transcriptional networks in each cell population are associated with tumor-mediated education, yet are also influenced by chromatin landscapes established before tumor initiation. Furthermore, we demonstrate that microglia specifically repress Itga4 (CD49D), enabling its utility as a discriminatory marker between brain-resident microglia and peripherally-derived macrophages in both primary and metastatic disease in mouse and human.
Project description:Transcriptional profiling of calpain-6-deficient murine bone marrow-derived macrophages comparing with calpain-6 wild-type macrophages. Total RNA was extracted from the pooled cells. Two-condition experiment, wild-type macrophages vs. calpain-6-deficiennt macrophages. The cells were derived from four mice, and were pooled for analysis.