Project description:This study aimed at characterizing the targets of the bZIP transcription factor Yap7 in two yeast species: Saccharomyces cerevisiae and the human pathogen Candida glabrata. Transcriptome analyses were thus conducted in wild type and yap7 knock out strains using Agilent microarrays.
Project description:Transcriptional profiling of Saccharomyces cerevisiae cells comparing the W303-1A wildtype with the W303-1A double mutant for MSN2 and MSN4 during zinc deficient conditions Keywords: Genetic modification with zinc limitation Two condition experiment, W303-1A vs W303-1A delta MSN2, MSN4. Biological replicates: 2 wildtype, 2 knock-out, independently grown and harvested.
Project description:Aims: We performed an analysis of maltotriose utilization by 52 Saccharomyces yeast strains able to ferment maltose efficiently and correlated the observed phenotypes with differences in the copy number of genes possibly involved in maltotriose utilization by yeast cells. Methods and Results: The analysis of maltose and maltotriose utilization by laboratory and industrial strains of the species Saccharomyces cerevisiae and Saccharomyces pastorianus (a natural S. cerevisiae/Saccharomyces bayanus hybrid) was carried out using microscale liquid cultivation, as well as in aerobic batch cultures. All strains utilize maltose efficiently as a carbon source, but three different phenotypes were observed for maltotriose utilization: efficient growth, slow/delayed growth and no growth. Through microarray karyotyping and pulsed-field gel electrophoresis blots, we analysed the copy number and localization of several maltose-related genes in selected S. cerevisiae strains. While most strains lacked the MPH2 and MPH3 transporter genes, almost all strains analysed had the AGT1 gene and increased copy number of MALx1 permeases. Conclusions: Our results showed that S. pastorianus yeast strains utilized maltotriose more efficiently than S. cerevisiae strains and highlighted the importance of the AGT1 gene for efficient maltotriose utilization by S. cerevisiae yeasts. Significance and Impact of the Study: Our results revealed new maltotriose utilization phenotypes, contributing to a better understanding of the metabolism of this carbon source for improved fermentation by Saccharomyces yeasts.
Project description:Proteins from within the Saccharomyces cerevisiae Mediator transcription complex were knocked out and compared against wild type yeast using two-color oligo arrays.
Project description:The goal of this experiment is to identify transcripts regulated by Cbc2, the small subunit of nuclear cap binding complex (CBC) in Saccharomyces cerevisiae. Experiment Overall Design: Six independent expression profiling experiments were carried out for isogenic wild-type (Y255) and cbc2Î (Y2685) strains using Affymetrix Yeast Genome S98 Arrays. Three repeats were applied to each strain.
Project description:We did transcription profiling on the effect of Zymolyase in Saccharomyces cerevisiae using strains BY4741 (wild type). Yeast cells exposed to Zymolyase in YPD growth medium show a significant induction of cell wall compensatory mechanism. Keywords: cell wall stress response
Project description:Proanthocyanidins (PAs) could pose significant enhancement on the metabolism and fermentation efficiency of Saccharomyces cerevisiae yeast cells, which has been elucidated from metabolic level and cell phenotype in our previous work. The goals of this study are to compare the transcriptome profiling (RNA-seq) of yeast cells treated with and without PAs to explore the possible global protection mechanism. After treated with 0 g/L, 0.1g/L, 1.0 g/L PAs, the total RNA of Saccharomyces cerevisiae strain AWRI R2 was extracted and sequenced at Illumina HiSeq sequencing platform. The analysis yielded a total of 524025062 clean sequencing reads. The clean reads were processed for the following bioinformatics analysis.
Project description:We did transcription profiling on the effect of O-glycosylation inhibitor OGT2468 in Saccharomyces cerevisiae using strains SEY6210 (wild type). Yeast cells exposed to OGT2468 in YPD growth medium show a significant inhibition of mating, filamentation and induction of cell wall compensatory mechanism.