Project description:This entry contains the proteomic files from a data set of RNAs, proteins, metabolites and lipids analyzed from the same staged samples of S. cerevisiae cells across the cell cycle. Actively growing (un-arrested) diploid cells were collected by elutriation as 8 distinct size pools across the cell cycle in triplicate. Yeast in these 24 aliquots were lysed and soluble and insoluble proteins were prepared for shotgun LC-MS/MS mass spectrometry analysis.
Project description:For the first time in any system, we generated experiment-matched datasets of the levels of RNAs, proteins, metabolites, and lipids from un-arrested, growing, and synchronously dividing yeast cells.
Project description:We use Saccharomyces cerevisiae to perform absolute quantitative multi-omics analysis to map interactions of different cellular processes during the yeast cell cycle.
Project description:We use Saccharomyces cerevisiae, grown on glucose and synchronized with CDC15-2, to map interactions of different cellular processes during the yeast cell cycle.
Project description:We use Saccharomyces cerevisiae grown on ethanol to perform absolute quantitative multi-omics analysis to map interactions of different cellular processes during the yeast cell cycle.
Project description:Dynamins are large multidomain GTPases involved in membrane scission. We have investigated the transcriptional consequences of deleting Vps1, a DNM2 homologue, in the yeast Saccharomyces cerevisiae. Interestingly, the vps1∆ mutation causes strong transcriptional defects also in yeast. Data suggest that DNM2 and Vps1 play an essential regulatory role connecting several membrane-related processes including endocytosis, lipid homeostasis and different signal transduction pathways.
Project description:RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.