Subcellular visualization and quantification of cyanotoxins microcystin or anabaenopeptin synthesis in bloom-forming cyanobacteria shows a distinct compartmentation

ABSTRACT: In this study time-lapse experiments have been performed using pulsed feeding of non-natural amino acids (non-AAs) in order to observe the build up or decline of azide- or alkyne-modified (clickable) microcystins (MCs) or anabaenopetins (APs) in M. aeruginosa and P. agardhii. Clickable MCs or APs have been subsequently labeled via Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) with ALEXA Fluor 488 (A488) and visualized using confocal microscopy and advanced image analysis using Imaris 10.1 surfaces function

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PROVIDER: S-BIAD1326 | bioimages |

REPOSITORIES: bioimages