Benchmark light microscopy images matching TEM images

ABSTRACT: Light Microscopy Imaging Workflow Resin-embedded tissue blocks were imaged using a custom-built imaging chamber. Low-magnification imaging datasets (Block FMs) were acquired with an air objective and DIC optics (Surface DIC map), utilizing 405, 488, 543, and/or 650 nm laser lines. Higher-resolution imaging datasets (ROI FMs) were collected with oil- or silicon-immersion objectives. Ultramicrotome camera images of the blocks were also captured. Ultrathin sections (200–300 nm) were prepared on glass slides or slot grids, placed in PBS, and imaged on an inverted Nikon AX-R NSPARC confocal microscope (Section FMs). File Naming Convention Format: {ExperimentID}_{AcquisitionDate}_{Microscope}_{SampleType}_{ObjectiveLens}.{FileType} ExperimentID: Unique identifier for the experiment AcquisitionDate: Date of image acquisition (YYMMDD) Microscope: A1: Nikon A1 confocal microscope AX: Nikon AX confocal microscope SoRa: Nikon SoRa spinning disk confocal microscope DIC: Transmitted light image acquired with a Nikon confocal microscope UC7: Leica UC7 ultramicrotome SampleType: Blk: Entire block Reg: Block region Section: Tissue section ObjectiveLens: 40x: 40× air objective 20x1x: 20× air objective, 1× zoom 25xOil: 25× silicon oil-immersion objective FileType: nd2: Nikon image format tif: Image file png: UC7 camera view

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PROVIDER: S-BIAD2077 | bioimages |

REPOSITORIES: bioimages