Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time

ABSTRACT: High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial  scattering and autofluorescence in tissue at visible (350–700?nm) and near-infrared (700–1,000?nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000–2,000?nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel det

SUBMITTER: Emily D. Cosco 

PROVIDER: S-BIAD27 | bioimages |

SECONDARY ACCESSION(S): https://gitlab.com/brunslab/ccda

REPOSITORIES: bioimages