Spatial genomics maps the structure, character and evolution of cancer clones

ABSTRACT: Genome sequencing of cancers often reveals mosaics of different subclones present in the same tumour. While these are believed to arise according to the principles of somatic evolution, the exact spatial growth patterns and underlying mechanisms remain elusive. To address this need, we developed a workflow that generates detailed quantitative maps of genetic subclone composition across whole tumour sections. These provide the basis for studying clonal growth patterns, and each clone’s histological characteristics, microanatomy, and microenvironmental composition. The approach rests on whole genome sequencing, followed by highly multiplexed base specific in-situ sequencing (BaSISS), single cell resolved transcriptomics and dedicated algorithms to link these layers. Applying the BaSISS workf

ORGANISM(S): Homo sapiens (human)

SUBMITTER:  

PROVIDER: S-BIAD537 | bioimages |

REPOSITORIES: bioimages