24h time series of U-2 OS knock-in cells expressing fluorescent fusion proteins of CRY1 and/or PER2, left untreated or after shRNA mediated knock-down of either CRY1 or PER2, respectively.

ABSTRACT: U-2 OS knock-in cells were genereated using CRISPR to express CRY1 and/or PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. Cells were either left untreated or trasnduced with lentivirus expressing shRNA against either PER2 or CRY1 (pGIPZ clones V2LHS_172866 (CRY1) V2LHS_52938 (PER2) with mutated tGFP). Cells were synchronized by washing them in cold PBS. Imging was performed on a Nikon widefield microscope for 24 h with an sampling interval of 1/h, starting 2.5 h after synchronization. Files are .nd2 and contain 4 channels:(1)RFP channel, (2)YFP channel, (3) a YFP/RFP channel (not used) (4) DIC channel. All files are stacks with 24 timepoints. nd2 file image stacks can be opened using the freeware Fiji/ImageJ, but also contain alot of metadata. For each cell line and condition, up to 5 imaging regions are provided. The cells identifiers are as follows: 42: CRY1-mClover3, 44: CRY1-mScarlet-I, 56: PER2-mClover3. 58: PER2-mScarlet-I. 80: CRY1-mClover3/PER2-mScarlet-I. More information on data acquisition and analysis can be found in the manuscript (doi provided after final publication)

ORGANISM(S): Homo sapiens (human)

SUBMITTER: Christian Gabriel 

PROVIDER: S-BSST631 | bioimages |

REPOSITORIES: bioimages