Comparative genomic hybridization by array of Campylobacter jejuni invasion of intestinal epithelial cells under low oxygen co-culture conditions that reflect the in vivo environment

ABSTRACT: Campylobacter jejuni infection often results in bloody, inflammatory diarrhea, indicating bacterial disruption and invasion of the intestinal epithelium. Whilst C. jejuni infection can be reproduced in vitro using intestinal epithelial cell (IEC) lines, low numbers of bacteria invading IECs do not reflect these clinical symptoms. Performing in vitro assays under atmospheric oxygen conditions is neither optimal for microaerophilic C. jejuni nor reflects the low oxygen environment of the intestinal lumen. A Vertical Diffusion Chamber (VDC) model system creates microaerobic conditions at the apical surface and aerobic conditions at the baso-lateral surface of cultured IECs producing an in vitro system that closely mimics in vivo conditions in the human intestine. Nine-fold increases in intera

ORGANISM(S): Campylobacter jejuni

SUBMITTER: Adam Witney 

PROVIDER: E-BUGS-125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress