Malaria Cell Atlas: Single cell transcriptomics of malaria parasites

ABSTRACT: Single-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations but has not yet been widely applied to single-celled organisms. Transcriptional variation in unicellular malaria parasites from the Plasmodium genus is associated with critical phenotypes including red blood cell invasion and immune evasion, yet transcriptional variation at an individual parasite level has not been examined in depth. Here, we describe the adaptation of a single-cell RNA-sequencing (scRNA-seq) protocol to deconvolute transcriptional variation for more than 500 individual parasites of both rodent and human malaria comprising asexual and sexual life-cycle stages. We uncover previously hidden discrete transcriptional signatures during the pathogenic part of the life cycle,

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Plasmodium falciparum 3D7

SUBMITTER: Array Express 

PROVIDER: E-ERAD-611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress