Transcription profiling of Drosophila expressing the tetanus toxin light fragment (TET) throughout the nervous system

ABSTRACT: Background: Synaptic transmission is required for functional maturation of the CNS and to induce gene transcription involved in neuronal plasticity. Our aim is to identify genes that are transcriptionally regulated by synaptic transmission during development. cDNA microarrays will be used to compare gene expression in normal embryos and embryos with no synaptic transmission. Using the Gal4 UAS system, the tetanus toxin light fragment (TET) will be expressed throughout the nervous system. The effects of TET are well characterised: the synaptic protein n-Synaptobrevin is cleaved, blocking evokes vesicle fusion in TET expressing neurons and as a result synaptic transmission is blocked. Embryos expressing an inactive, mutant version of TET (iTET) show no detectable phenotype. Thus a comparison

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Debashis Rana 

PROVIDER: E-FLYC-2 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress